Objective To compare T-SPOT. TB and Enzyme-linked immunosorbent assay(ELISPOT) using ESAT-6/CFP-10 fusion proteinas( E/C) and Rv3879c as antigens,and explore their roles in the auxiliary diagnosis of active tuberculosis. Methods Totally 106 patients with initial pulmonary tuberculosis(tuberculosis group) and 48 healthy person(healthy control group) and 43 patients with non-tuberculosis pulmonary disease(disease control group) were enrolled. The peripheral blood mononuclear cells of all participants were detected by ELISPOT and T-SPOT. TB, Then enumerated the spot forming cells(SFCs). All participants were also performed TST simultaneously. Results The sensitivity of ELISPOT and T-SPOT. TB were 90.6% and 93.4% in tuberculosis group and specificity were 91.5% in TST negative control group. The positive rate were 18. 8% and 16.7% in healthy person by these two methods, There wre not statistically significance difference between two methods(P >0.05). Conclusions Enzyme-linked immunosorbent assay( ELISPOT) using ESAT-6/CFP-10 fusion proteinas( E/C) and Rv3879c as antigens can be a assistant to diagnosis active tuberculosis. It offers certain diagnostic evidences for latent tuberculosis infection.%目的 比较E/C联合Rv3879c蛋白作为抗原的ELISPOT和T-SPOT.TB试剂盒,探讨其在活动性结核病诊断中的应用价值.方法 选取初治结核病患者106例作为结核组,43例肺部其它疾病患者做为疾病对照组和48例健康者做为健康对照组,应用T-SPOT.TB和ELISPOT检测受试者外周血单个核细胞的斑点形成细胞数量,同时对受试者行T研.结果 T-SPOT.TB和ELISPOT在结核病组中的敏感度分别为92.5％和93.4％,TST阴性对照组中的特异度均为91.5％,在健康者中的阳性率分别为18.8％和16.7％,且两种方法差异无统计学意义(均P＞0.05).结论 E/C联合Rv3879c蛋白作为抗原的ELISPOT能够诊断活动性结核病,并为结核感染诊断提供一定的依据.
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