肺癌、贲门癌及食管癌外周血的免疫调节因子及相关抗原自身抗体表达研究

摘要

Objective To investigate the effect and interaction of immune regulatory factors and tumor-asso-ciated antigens in peripheral blood of lung cancer,cardia carcinoma and esophageal cancer,in order to provide theo-retical basis for early diagnosis of tumors. Methods In this study,it selected 7 molecular(including 3 immunoregu-latory factors)and 4 tumor -related antigens. It used bioinformatics software to design peptide antigen fragments of 7b molecular,and ELISA method to detect peripheral blood of esophagus carcinoma and peptide antigen antibody of NSCLC. 200 healthy volunteers were taken as the control group,and their mixed blood was taken as the quality con-trol samples. It used SPSS18. 0 data processing software to do analysis,and P < 0. 05 meant statistical significance. Results Compared with the control group,the level of anti-CD25IgG showed significance in adenocarcinoma of lung and adenosquamous carcinoma,and it was lower in the control group than in the lung cancer group(P < 0. 05). The positive rate of ESCC and NSCLC was higher than that of the control group. The study showed that the level of anti-CD25IgG was helpful for the early diagnosis of ESCC and NSCLC. The level of anti-P16IgG was higher in patients with lung cancer,cardia carcinoma and esophageal cancer than in the control group,but it showed no significant clin-ical diagnostic value. Conclusion Anti-CD25IgG and anti-FOXP3IgG in peripheral blood of patients with lung canc-er,cardia carcinoma and esophageal cancer can provide evidence for clinical diagnosis. The level of anti - P16IgG was correlated with lung cancer,cardia carcinoma and esophageal cancer,but its positive rate is not obvious. The clinical diagnostic value needs to be in-depth study.%目的：肺癌、贲门癌、食管癌是胸部主要肿瘤，具有高发病率和死亡率的特点，早期发现和治疗肺癌、贲门癌及食管癌意义重大，本研究旨在探讨免疫调节分子及肿瘤相关抗体在肿瘤发病中的作用及相互影响，为发现肿瘤早期诊断的标志物提供理论依据。方法本研究中，选择7个分子（含3个免疫调节因子）及4个肿瘤相关抗原。使用生物信息学数据库对蛋白质结构和性质进行综合分析，并通过生物信息学软件对7个分子的多肽抗原片段进行设计。采用 ELISA 法对食管癌患者检测外周血及非小细胞肺癌患者多肽抗原的抗体水平，选择健康体检者为对照组，采用对照组超过250例健康体检者的混合血作为质控样本。使用SPSS18.0数据处理软件对相关数据进行统计学分析，卡方检验，（P <0.05）具有统计学意义。结果与健康体检者水平相比，肺腺癌及肺鳞癌外周血 anti-CD25IgG 水平存在统计学意义（P <0.05），对照组男女健康体检者 anti-CD25IgG 水平明显低于肺癌患者。anti-CD25IgG 检测中，ESCC 及 NSCLC 患者阳性率较对照组明显偏高。实验结果表明，诊断早期 ESCC 和 NSCLC，anti-CD25IgG 水平有助于提高其诊断性。抗体检测：肺癌、贲门癌及食管癌患者 anti-P16IgG 在检测中，其水平是明显高于对照组的，但其临床诊断意义不明显。结论在肺癌、贲门癌及食管癌患者外周血中，anti-CD25IgG 及anti-FOXP3IgG 能为临床诊断提供依据；肺癌、贲门癌及食管癌与anti-P16IgG 水平的改变具有相关性，但其阳性率不明显，其临床诊断价值有待深入研究。