诱导HO-1表达对慢性间歇性缺氧下INS-1细胞凋亡的影响

摘要

目的 了解诱导HO-1表达对慢性间歇性缺氧下INS-1细胞凋亡的影响.方法 通过不同浓度CoPP作用于慢性间歇性缺氧下INS-1细胞,观察胰岛素水平、HO-1及Caspase-3水平,采用RT-PCR检测HO-1、Caspase-3mRNA表达.结果 随着CoPP浓度增高,胰岛素、HO-1表达逐渐增高,而Caspase-3逐渐降低,而CoPP浓度达到40umol/L时作用最强(P<0.05).结论 慢性间歇性缺氧下诱导INS-1细胞HO-1表达,抑制凋亡相关蛋白Caspase-3,在一定程度上对细胞起保护作用.%Objective To understand the effect of HO-1 expression on the apoptosis of INS-1 cells induced by chronic intermittent hypoxia.Methods INS-1 cells were observed under different concentrations of CoPP in chronic intermittent hypoxia, and the levels of insulin were observed.It used RT-PCR to detect the expression of HO-1 and Caspase-3 mRNA.Results With the increase of CoPP concentration, the expression of insulin and HO-1 mRNA gradually increased, while Caspase-3 mRNA decreased.When the concentration of CoPP reached 40umol/L, the strongest effect was observed.Conclusion The induced expression of HO-1 in INS-1 cells can inhibit protein Caspase-3, and protected the cells at certain extent.