Objetive To characterize the expression and significance of PDCD5 gen in bile duct tissues of Biliary Atresia(BA)and Choledochal Cyst(CC). Methods Small pieces of bile duct tissues were obsta-ined from 1 8 patients with BA and 1 5 patients with CC,expression of PDCD5 from Protein level and Gene level was detected by Weston blot and RT-PCR. Results In the BA patients,the Cycle threshold(CT)of PDCD5 in bile duct tissues was(4.0125 ±1.0735),which lower than the CC patients (6.7292 ±1.169)(P<0.05). The ratio of PDCD5 in CC and BA was 1 ∶6.by 2-ΔΔCT.Above all,expression of PDCD5 from Gene level was enhanced in bile duct tissues of BA;And western blotting,result in the BA patients,expression of PDCD5 from Protein level was higher than CC patients. Conclusion Expression of PDCD5 from Protein level and Gene level were enhanced in bile duct tissues of BA.It is Probably that PDCD5 may be involved in the bile duct epithelia cell apoptosis of BA and participate in BA inflammatory reaction,which play an important role in the pathogenesis of BA.%目的:研究促凋亡基因PDCD5在胆道闭锁及胆总管囊肿患儿胆管组织中的表达规律,探讨促凋亡基因PDCD5在胆道闭锁发病机制中的作用。方法分别取18例胆道闭锁(Biliary Atre-sia,BA)和11例胆总管囊肿(Choledochal cyst,CC)患儿的胆管组织,应用蛋白质印迹(westong blot)与荧光实时定量(quantitative real-time PCR,qRT-PCR)的方法分别检测PDCD5在胆道闭锁与胆总管囊肿患儿胆管组织中的表达,并进行定位和定量分析与比较。结果 PDCD5在CC组胆管组织中的CT值为(6.7292±1.169),高于BA组的相应CT值(4.0125±1.0735),P<0.05。应用2-ΔΔCT计算出CC 与BA中PDCD5基因表达量的比值为1∶6.57。PDCD5在BA组胆管组织中mRNA转录水平明显高于对照组;Western b1 ot结果显示,PDCD5在BA组胆管组织中蛋白表达量明显高于CC组。结论 PDCD5在胆道闭锁患儿胆管组织中的蛋白和基因表达水平均增强。PDCD5可能参与胆道闭锁胆管上皮细胞凋亡,从而参与胆道闭锁的炎性反应,在胆道闭锁的发病机制中发挥重要作用。
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