长链非编码 RNA SPRY4-IT1在脑胶质瘤中的表达及对 U87细胞功能的影响

摘要

Objective To investigate the SPRY4-IT1 expression levels in glioma and the role of SPRY4-IT1 in glioma U87 cell biological functions .Methods Quantitative real-time polymerase chain reactions for SPRY4-IT1 expression were performed in 40 pairs of glioma tissues and adjacent tissues in our department .Lentiviral-mediated SPRY4-IT1 knockdownin U87 was further to investigate its role in glioma cell biological functions .Cell growth, apoptosis and migration were tested in vitro by CCK-8 assays, flow cytometry and transwell assays ,respectively.Results SPRY4-IT1 expression was significantly overexpressed in cancer tissues (P<0.05).Knockdown of SPRY4-IT1 expression reduced cell migration(P<0.05), but have no significant changes in cell growth (P>0.05) and cell apoptosis ( P >0.05).Conclusions SPRY4-IT1 was overexpressed in glioma tissues and promoted the migration of U87 cells.The expression of long non-coding RNA SPRY4-IT1 may play an important role in the process of glioma development and may be developed as a useful biomarker for the diagnosis and prognosis of glioma .%目的：探讨长链非编码RNA SPRY4-IT1在脑胶质瘤中的表达，以及对胶质瘤细胞系U87细胞生物学功能的影响。方法收集我科40例胶质瘤手术标本（包括肿瘤组织和瘤周组织），荧光实时定量PCR （ qRT-PCR）检测40对标本中SPRY4-IT1的表达情况；用慢病毒介导shRNA干扰SPRY4-IT1表达后，分别采用CCK-8法、流式细胞仪和Transwell法检测其对细胞增殖、凋亡和迁移的影响。结果 SPRY4-IT1在肿瘤组织中的表达量为11．73±0．55，高于瘤周组织的9．14±0．59，差异有统计学意义（P＜0．05）。 SPRY4-IT1表达被干扰后，胶质瘤细胞株U87迁移能力明显降低，差异有统计学意义（ P＜0．05），但细胞增殖无明显变化，差异无统计学意义（P＞0．05），细胞凋亡变化亦不明显，差异无统计学意义（P＞0．05）。结论 SPRY4-IT1在胶质瘤组织中高表达，并且能促进U87细胞迁移，可能成为胶质瘤诊断和判断预后的重要分子标记物。