Objective To explore the plasma level of aldosterone( Ald) and the tissue mRNA expression of aldosterone - synthase ( CYP11B2 ) in patients with or without atrial fibrillation ( AF ). Methods Thirty - eight patients who underwent opened thoracic surgery were enrolled. They were di vided into 3 groups according to with or without history of AF: Sinus Rhythm group, Paroxysmal AF group and Chronic AF group. The plasma concentrations of Ald were determined by the method of ra dio - immunity assay, and the mRNA expression of CYP11 B2 in atrial myocardium was determined by using Semi - Polymerase Chain Reaction ( Semi - PCR). Results Plasma Ald levels were significant ly higher in cAF group than pAF group and SR group (P<0.01 ). CYP11B2/GAPDH gray ratio was significantly higher in pAF group than the SR group (P <0. 01) , significantly lower than cAF group (P <0.05). Plasma Aid circulating concentration and expression of atrial tissue CYP11B2mRNA and left atrial diameter were significantly correlated with changes in the relationship (P<0.01). Conclu sion Ald may probably participate in the development and persistent of atrial fibrillation through me diating atrial structural remodeling.%目的 分析心房颤动患者循环血浆醛固酮( Ald)水平和心房肌组织中醛固酮合成酶(CYP11 82)的mRNA表达情况,探讨其在心房颤动发病中的作用.方法 38例因心脏疾患住院接受开胸手术的患者,分为窦性心律(SR)组,阵发房颤(pAF)组和慢性房颤(cAF)组.放射免疫方法测量循环血浆醛固酮水平,半定量聚合酶链式反应技术测定CYP11B2mRNA在心房肌组织中的表达情况.结果 cAF组血浆Ald浓度明显高于pAF组与SR组(P＜0.01)；pAF组CYP11B2/GAPDH灰度比值较SR组明显升高(P＜0.01),较cAF组明显降低(P＜0.05).循环血浆Ald浓度和心房肌组织CYP11B2mRNA表达水平分别与左心房直径变化具有明显的正相关关系(P＜0.01).结论 Ald可能通过介导心房结构重构,参与心房颤动的发生和维持.
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