β-咔啉类生物碱对人胃癌细胞SGC-7901增殖的影响

摘要

目的：探讨β-咔啉类生物碱对人胃癌细胞 SGC-7901增殖的影响。方法通过体外培养人胃癌细胞株 SGC-7901，将含有不同浓度（5、10、20、40μg/mL）β-咔啉类生物碱的培养液与 SGC-7901细胞共同培养24 h 和48 h。采用 MTT 比色法计算细胞抑制率；在荧光显微镜下用 Hoechst 33258细胞核染色法观察细胞形态学改变；流式细胞仪检测凋亡率及基因组DNA 琼脂凝胶电泳检测凋亡梯状条带。结果β-咔啉类生物碱对 SGC-7901细胞的损伤呈浓度依赖性；β-咔啉类生物碱分别作用24 h 和48 h 后半数抑制浓度（IC50）分别为17．79μg/mL 和12．17μg/mL；荧光染色可观察到有细胞核固缩及核断裂的凋亡现象；流式细胞术显示：阴性对照组（0μg/mL）及β-咔啉类生物碱5、10、20、40μg/mL 浓度组24、48 h 凋亡率为别1．66％、11．27％、20．32％、30．66％、41．42％和3．84％、15．29％、23．34％、34．87％、49．54％，细胞凋亡率伴随给药浓度增加而增加；基因组 DNA 琼脂糖凝胶电泳检测到明显的凋亡梯状条带。结论β-咔啉类生物碱能诱导人胃癌 SGC-7901细胞发生凋亡，抑制细胞增殖。%Objective To investigate the effects of β-Carboline of alkaloids on proliferation of SGC-7901 of human gastric cancer cells.Methods SGC-7901 cells were cultured in vitro initially. After SGC-7901 cells were incubated with β-Carboline alkaloids at different concentrations of 5,10, 20,40 μg/mL for 24,48 hours,the inhibited proliferation rate of SGC-7901 cells were examined by Methtl Thiazolyl Tetrazolium(MTT)assay.Morphological changes of SGC-7901 cells were observed by Hoechst 33258 under fluorescence microscope.Flow cytometry was used to detect cell apoptosis after SGC-7901 cells were incubated with β-Carboline alkaloids for 24 hours.Cell gemonic DNA was detec-ted by agarose electrophoresis.Results β-Carboline of alkaloids induced damage of SGC-7901 cell in a concentration dependent manner .The IC 5 0 of β-Carboline alkaloids at 2 4 ,4 8 hours were 17.79 μg/mL and 12.17 μg/mL respectively.Apoptotic cells were observed and flow cytonetry analy-sis in dicated that the apoptosis rate of cells treated with different concentrations of β-Carboline alka-loids(0,5,10,20,40 μg/mL)for 24 and 48 hours were 1.66%,11.27%,20.32%,30.66%, 41.42%;3.84%,15.29%,23.34%,34.87%,49.54%,respectively.Increasing in apoptosis rate of SGC-7901 cells was associated with drug concentration.Typical DNA Ladder was detected in DNA agarose electrophoresis.Conclusion β-Carboline alkaloids can inhibit the proliferation and in-duce the apoptosis of SGC-7901 cells.