非小细胞肺癌组织中 Napsin-A 表达与EGFR 基因的相关性研究

摘要

Objective To explore the relationship between Napsin-A expression and epider-mal growth factor receptor (EGFR)genes in non-small cell lung cancer (NSCLC)tissue.Methods Napsin-A expression in 89 adenosquamous carcinoma tissues of the lung was detected by immunohisto-chemistry,and the expression of EGFR genes in the tissues above was also detected by ARMS kit.The relationship between Napsin-A expression and EGFR was analyzed.Results The Napsin-A positive rates of adenocarcinoma and squamous carcinoma in NSCLC tissue were 96.2% and 5.6%,respec-tively,and there was statistical significance between them.The mutation rate of EGFR gene in lung adenocarcinoma tissue was 54.7%,while that of the patients with positive Napsin-A expression came up to 100.0%.The mutation rate of EGFR gene in lung squamous tissue was 5.6%,while that of the patients with positive Napsin-A expression came up to 50.0%,in which significant difference was presented by comparison to them.Conclusion Napsin-A is highly expressed in lung adenocarcinoma tissue,and the EGFR mutation rate of patients with positive expression in adenosquamous carcinoma is also high.Hence,for the patients with positive Napsin-A expression,it should be recommended to de-tect EGFR genes.%目的：探讨非小细胞肺癌（NSCLC）组织中 Napsin-A 表达与表皮生长因子受体（EGFR）基因的关系。方法采用免疫组织化学法检测89例肺腺鳞癌组织中 Napsin-A 的表达情况，并用 ARMS 试剂盒检测上述组织中 EGFR 基因的表达情况，分析 Napsin-A 的表达与 EGFR 基因的关系。结果在 NSCLC 组织中，腺癌和鳞癌的 Napsin-A 阳性率分别为96．2％和5．6％，两者比较差异有统计学意义。肺腺癌组织中，EGFR 基因突变率为54．7％，而 Napsin-A 表达阳性者的突变率高达100．0％；肺鳞癌组织中，EGFR 基因突变率为5．6％，而 Napsin-A 表达阳性者的突变率为50．0％，两者比较，差异有统计学意义。结论Napsin-A 在肺腺癌组织中高表达，且在腺鳞癌中表达阳性者 EGFR 基因突变率较高，Napsin-A 表达阳性者可建议行 EGFR 基因检测。