茶多酚对人结肠癌细胞株 Caco-2凋亡及RhoA 蛋白活性的影响

摘要

目的：探讨茶多酚对人结肠癌细胞株 Caco-2凋亡及 RhoA 蛋白活性的影响。方法将传代后的人结肠癌细胞株Caco-2细胞分为实验组和对照组，实验组加入不同浓度的茶多酚（25、50、100和200μmol /L），对照组加入等量 RPMI-1640培养液，采用四甲基偶氮唑盐（MTT）法测定茶多酚对 Caco-2增殖抑制的影响，流式细胞术（FCM）检测细胞凋亡指数，Pull-down法检测人结肠癌细胞株 Caco-2细胞内的 RhoA 蛋白活性。结果不同浓度的茶多酚对人结肠癌细胞株 Caco-2有明显的抑制作用，且呈剂量及时间依赖性；Pull-down 法检测结果显示，茶多酚可以降低人结肠癌细胞株 Caco-2细胞内的 RhoA 蛋白活性，且随浓度增加其蛋白活性降低。结论茶多酚可促进人结肠癌细胞株 Caco-2的凋亡，其可能通过降低 RhoA 蛋白活性来实现。%Objective To explore the influence of tea polyphenols (TP)on the apoptosis of human colon cancer cell line Caco-2 and RhoA protein activity.Methods The passage human colon cancer cell line Caco-2 cells were divided into experimental group and control group.The experimental was added with different concentrations of TP (25,50,100 and 200 μmol /L),while the control group was added with equal amount of culture fluid RPMI-1640.Inhibition of Caco-2 proliferation,ap-optosis indexes and RhoA protein activity within human colon cancer cell line Caco-2 cells were re-spectively detected by methyl thiazolyl tetrazolium (MTT),flow cytometry (FCM)and Pull-down method.Results Different concentrations of TP showed significant inhibitory effects on human colon cancer cell line Caco-2.Pull-down test results showed that TP could reduce the activity of RhoA pro-tein in human colon cancer cell line Caco-2 cells,and with the increasing of concentration,its protein activity decreased.Conclusion TP can promote the apoptosis of human colon cancer cell line Caco-2, which may be achieved by reducing the activity of RhoA protein.