血清结肠癌转移相关基因-1在胃癌患者化疗疗效预测中的效果

摘要

Objective To explore the effect of serum MACC1 on forecasting chemotherapeutic effect of patients with gastric cancer.Methods The difference in sensitivity of BCG-823 to 5-fluorouracil (5-Fu) between gastric cancer cell line BCG-823 with over-expression and low expression of MACC1 was detected by MTT method.The expression of MACC1 mRNA in gastric cancer cells with over-expression and low expression was detected by RT-PCR.ELISA was used to detect the expression of MACC1 protein in MACC1 over-expressed and low expression cells.Relationship between the expression of MACC1 in peripheral blood before chemotherapy and the effect of chemotherapy in patients with advanced gastric cancer was analyzed.Results The mRNA expression level of MACC1 decreased after MACC1 silencing and increased after MACCI over-expression,which were consistent with the tendency of protein expression level.After interfering with MACC1,the sensitivity of BCG-823 to 5-Fu increased,and the sensitivity to 5-Fu reduced after over-expression of MACC1.The patients with low contents of serum MACC1 showed prior chemotherapeutic effect to those with high contents of MACC1.Conclusion MACC1 can be used as a useful biomarker for forecasting the efficacy of chemotherapy in patients with gastric cancer.%目的 探讨血清结肠癌转移相关基因-1(MACC1)对胃癌患者化疗疗效的预测作用.方法 MTT法检测MACC1过表达、低表达的胃癌细胞株BCG-823对5-氟脲嘧啶(5-Fu)敏感性的差异.RT-PCR检测MACC1过表达、低表达的胃癌细胞中MACC1 mRNA表达水平.ELISA法检测MACC1过表达、低表达细胞培养液中MACC1蛋白表达水平.分析晚期胃癌患者化疗前外周血中MACC1表达水平与化疗疗效的关系.结果 干扰MACC1后,BCG-823细胞中mRNA表达水平下降,过表达细胞中的mRNA表达水平上升,其趋势与细胞培养液中MACC1蛋白表达水平一致.干扰MACC1后,BCG-823对5-Fu敏感性增高,过表达MACC1后其对5-Fu敏感性降低.晚期胃癌患者MACC1蛋白含量较低的患者化疗疗效好于MACC1含量较高的患者.结论 MACC1能够影响胃癌病人对化疗的敏感性,可以有效预测患者化疗疗效.