不同HBV标志物模式的乙型肝炎患者HBV表面大蛋白的检测及与HBV DNA水平的相关性

摘要

Objective To investigate the relationship between HBV large envelop protein ( HBV - LP) and HBV DNA/patterns of HBV markers and the significances of HBV - LP in clinical diagnosis and treatment of HBV patients, the HBV - LP, preS1 and HBV DNA in HBV patients with different patterns of HBV markers(HBV M, HBsAg/HBsAb、HBeAg/HBeAb、HBcAb) were tested. Methods The HBV - LP, preS1 and patterns of HBV M were tested by ELISA, and HBV DNA was measured by fluorescence quantitative PCR. Results No significant difference was found between HBV - LP and HBV DNA level (x2 = 1.97, P ＞ 0. 05 ). There was a positive correlation with the value A of HBV - LP and the HBV DNA level ( r =0.565, P ＜0.01 ), and there was a significant difference of value A of HBV - LP in different copies of HBV DNA( F =9.23 ,P ＜0. 01 ). Conclusion The HBV - LP was a good sero - immunological marker to reflect the level of virus replication in HBV patients. The HBV - LP in serum has a good positive correlation with HBV DNA, and especially served as a good serum target in monitoring the virus replication and prognosis.%目的 通过检测乙型肝炎病毒标志物(HBV M:HBsAg/抗-HBs、HBeAg/抗-HBe、抗-HBc)不同模式的乙型肝炎患者血清中乙型肝炎病毒外膜大蛋白(HBV-LP)、乙型肝炎病毒前S1抗原(HBVpreS1)、HBV DNA,探讨HBV-LP与HBV DNA及HBV M模式间的关系,研究HBV-LP用于乙型肝炎患者临床诊治的意义.方法 采用酶联免疫吸附试验(ELISA)对HBV-LP、HBVpreS1和HBV M进行检测;采用荧光定量PCR方法对HBV DNA进行检测.结果 乙型肝炎患者大蛋白检测结果与HBV DNA 检测结果差异无统计学意义(X2=1.97,P>0.05).HBV-LPA值随HBV DNA拷贝数的增加呈上升趋势,二者之间存在良好的相关性(r=0.565,P<0.01),在不同HBV DNA拷贝数组别间,HBV-LP A值差异有统计学意义(F=9.23,P<0.01).结论 HBV-LP是反应HBV感染者体内病毒复制程度的良好的血清免疫学指标,血清中的HBV-LP与HBV DNA具有较好的相关性,特别是可作为HBeAg阴性患者体内监测病毒复制及预后判断的良好的血清学指标.