经沉默免疫负调控基因技术处理的树突状细胞联同细胞毒性T淋巴细胞对HepG2细胞移植瘤的抑制作用

摘要

目的：观察经沉默免疫负调控基因（iAPA）技术处理的树突状细胞（DC）联同细胞毒性T淋巴细胞（CTL）（iAPA-DC/CTL）对HepG2细胞移植瘤的抑制作用。方法利用人肝癌细胞系HepG2建立裸鼠皮下移植瘤模型，将12只裸鼠随机分为2组：生理盐水对照组（C组）和iAPA-DC/CTL组（DC组），每组6只，行iAPA-DC/CTL治疗4次（1周/次）后处死。实验期间观察各组裸鼠的肿瘤生长，测量肿瘤长短径并描绘肿瘤生长曲线，称量瘤重并计算抑瘤率，病理检测。两组间均数比较采用成组t检验。结果造模成功率为92．31％。C组和DC组肿瘤体积分别为：（697．69±143．99）、（485．64±188．75）mm3，DC组生长相对缓慢（t＝2．28，P＜0．05）；C组和DC组肿瘤重量分别为：（0．32±0．07）、（0．22±0．08）g，DC组肿瘤重量小于对照组（t＝2．31，P＜0.05），抑瘤率为30．39％。肿瘤免疫组化染色后T淋巴细胞计数分别为：C组未见、DC组（39．74±5．11）个/高倍视野，DC组肿瘤内T细胞数多于对照组（t＝19．05，P＜0．05）。结论 iAPA-DC/CTL能够有效抑制HepG2细胞裸鼠皮下移植瘤的生长。%Objective To investigate the inhibitory effect of inhibition of antigen presentation attenuators (iAPA)-based dendritic cells (DC)and cytotoxic T lymphocytes (CTL)(iAPA -DC/CTL)on HepG2 xenograft in nude mice.Methods Human hepatocarcinoma HepG2 cells were subcutaneously implanted into nude mice on nude mice to establish a HepG2 xenograft model transplanted tumor model of human HCC.Twelve nude mice were randomly and equally divided into two groups:normal saline control group (C group)and iAPA-DC/CTL group (DC group).After four times of treatment with iAPA-DC/CTL (once a week),all mice were sacrificed.Tumor growth was e-valuated by measuring the long and short diameters and delineating the tumor growth curve.The tumors were weighed,and the tumor inhibi-tion rate was calculated.In addition,histopathological examination was performed.Comparison of means between the two groups was made by independent-samples t test.Results The HepG2 xenograft model was successfully established in 92.31%of all mice.The tumor vol-ume was 697.69 ±143.99 mm3 in C group and 485.64 ±188.75 mm3 in DC group;the tumor growth was significantly slower in DC group than in C group (t=2.28,P<0.05).The tumor weight was 0.32 ±0.07 g in C group and 0.22 ±0.08 g in DC group;DC group had a significantly lower tumor weight than C group (t=2.31,P<0.05),and the tumor inhibition rate was 30.39%.After immunohistochemical staining,T lymphocyte count was 0 cell/high-power field (HPF)in C group and 39.74 ±5.11 cells/HPF in DC group;the number of T lymphocytes in DC group was significantly higher than that in C group (t=19.05,P<0.05).Conclusion iAPA-DC/CTL can effective-ly inhibit the growth of subcutaneous HepG2 xenograft in nude mice.