摘要：
目的 探讨微球联合碘油栓塞介入对肝癌大鼠肝细胞增殖、凋亡的影响.方法 将30只成功建立肝癌模型的雄性大鼠随机分为联合组(n=10)、碘油组(n=10)及模型对照组(n=10),分别经肝动脉注入0.5 ml/kg碘油±1 ml Embosphere微球、0.5 ml/kg碘油、1.5 ml/kg生理盐水,治疗8d后观察肿瘤生长率,TUNEL法检测大鼠肝癌细胞凋亡情况,免疫组化SP法检测肝癌组织中p53和Bcl-2表达情况.结果 3组肿瘤生长率对比差异具有统计学意义(均P＜0.05),联合组肿瘤生长率明显低于碘油组及模型对照组[(2.21±0.64)％比(10.68±3.08)％、(28.42±9.13)％,均P＜0.05].3组肝癌细胞凋亡率对比差异具有统计学意义(均P＜0.05),联合组肝癌细胞凋亡率明显高于碘油组及模型对照组[(19.87±5.32)％比(14.69±4.13)％、(3.72±1.24)％,均P＜0.05].p53和Bcl-2免疫组化阳性着色部位主要在胞质中,呈黄色或棕黄色颗粒;3组p53、Bcl-2阳性表达率对比差异具有统计学意义(均P＜0.05),联合组p53阳性表达率明显高于碘油组及模型对照组[(55.41±5.93)％比(41.38±6.42)％、(24.36±6.85)％,均P＜0.05],Bcl-2阳性表达率明显低于碘油组及模型对照组[(26.74±7.59)％比(41.38±6.42)％、(67.25±8.76)％,均P＜0.05].结论 微球联合碘油栓塞介入治疗能够抑制肝癌细胞增殖,促进细胞凋亡,其可能是通过上调p53蛋白表达、下调Bcl-2蛋白表达来实现的.%Objective To investigate the effects of microspheres combined with lipiodol embolization on hepatocyte proliferation and apoptosis in rats with liver cancer.Methods Thirty successfully established male rat models with liver cancer were randomly divided into the combination group,lipiodol group and model control group (n=10 each).The three groups were injected with 0.5 ml/kg lipiodol +1 ml Embosphere,0.5 ml/kg lipiodol,and 1.5 ml/kg normal saline through hepatic artery,respectively.At 8 d after the treatment,the tumor growth rate was determined.The apoptosis of liver cancer cells was examined by TUNEL method.Immunohistochemical SP method was used to determine the expression of p53 and Bcl-2 in liver cancer tissues.Results There was statistically significant difference in the tumor growth rate among the three groups (all P＜0.05).The tumor growth rate in the combination group was significantly lower than that in the lipiodol group and model control group [(2.21±0.64)％ vs (10.68±3.08)％,(2.21 ±0.64)％ vs (28.42±9.13)％,both P＜0.05].There was statistically significant difference in the apoptosis rate of liver cancer cells among the three groups (all P＜0.05).The apoptosis rate of liver cancer cells in the combination group was significantly higher than that in the lipiodol group and model control group [(19.87±5.32)％ vs (14.69±4.13)％),(19.87±5.32)％ vs (3.72± 1.24)％,both P＜0.05].The immunohistochemical positive staining sites of p53 and Bcl-2 were mainly in the cytoplasm,showing yellow or brown granules.There were statistically significant differences in the positive expression rates of p53 and Bcl-2 among the three groups (all P＜0.05).The positive expression rate of p53 in the combination group was significantly higher than that in the lipiodol group and model control group [(55.41±5.93)％ vs (41.38±6.42)％,(55.41±5.93)％ vs (24.36 ± 6.85)％,both P＜0.05].The positive expression rate of Bcl-2 in the combination group was significantly lower than that in the lipiodol group and model control group [(26.74±7.59)％ vs (41.38± 6.42)％,(26.74±7.59)％ vs (67.25±8.76)％,both P＜0.05].Conclusion Microspheres combined with lipiodol embolization may inhibit the proliferation of liver cancer cells and promote cell apoptosis,which may be achieved by up-regulating the protein expression of p53 and down-regulating the protein expression of Bcl-2.
