Objective To investigate the effect of miRNA-384 (miR-384)expression on hepatic steatosis in mice with nonalcoholic fatty liver disease (NAFLD)induced by high-fat diet (HFD). Methods A total of 30 male C57BL/6J mice were fed for 7 days to adapt to the environment and then randomly divided into 2 groups,with 15 mice in each group. The mice in the control group were given normal diet, and those in the model group were given HFD for 8 weeks and then the liver tissue was harvested. HE and Nile red staining were used to ob-serve the pathological changes of the liver. Microarray sequencing was performed to determine the whole-genome miRNA expression profile of liver tissue,and PCR was used to measure the relative expression of miR-384. The t-test was used for the comparison of continuous da-ta between groups. Results In the control group,the liver was red with sharp edges,the lobular structure was clear,and there was no he-patic steatosis;in the model group,the liver was yellow with blunt edges,and the hepatocytes were swollen with a large number of fat vacu-oles in the cytoplasm and nuclear deviation caused by the fusion of lipid droplets. Compared with the normal mice,the NAFLD mice had 12 upregulated miRNAs and 18 downregulated miRNAs in liver tissue. Some of the differentially expressed miRNAs between the control group and the model group were screened to obtain the same cluster diagram. Among the 8 miRNAs with significant changes,miR-384 showed a significant fold change. Conclusion The upregulation of miR -384 is closely associated with hepatic steatosis,but its mechanism still needs further study.%目的 研究microRNA(miRNA)-384的表达对高脂饮食诱导非酒精性脂肪性肝病(NAFLD)小鼠肝脂肪变性的影响.方法 将30只雄性C57BL/6J小鼠适应性喂养7 d,然后随机分成2组,每组15只.对照组中的15只小鼠给予普通饮食,模型组15只小鼠给予HFD,喂养8周后获取肝组织.进行HE和尼罗红染色观察肝组织病理改变.采用微阵序列测序肝组织的全基因组miRNA表达谱,PCR法测定miRNA-384的相对表达水平.计量资料2组间比较采用t检验.结果 对照组小鼠肝脏呈红色,边缘锐利,肝小叶结构清晰,肝细胞无脂肪变性;模型组小鼠肝脏呈黄色,边缘变钝,肝细胞明显肿胀,细胞质内可见大量脂肪空泡,脂滴相互融合导致细胞核偏位.与正常小鼠相比,在NAFLD小鼠的肝组织中发现了12个上调和18个下调的miRNA.筛查了对照组和模型组间的一些差异表达的miRNA,获得同样的聚类分析图.在这8个明显改变的miRNA中,miRNA-384具有显著的倍数变化.结论 miRNA-384表达的上调与肝脏脂肪变性有着密切关系,但是其作用机制仍需进一步研究.
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