Objective To study the impact of lenalidomide( LEN ) on KM3 eels from multiple myeloma patients( MM ) and its mechanism. Methods The effect of lenalidomide on the KM3 cell growth inhibition was detected by the means of trypan blue staining, and the cells ap-optosis was detected by the means of flow cytometry ( Annexin - V/FFTC method ). Results After different concentratios of lenalidomide acted in KM3 cells for 24hrs, the KM3 cell survival percentage gradually decreased, were 100.0% ±2.5%, 87.2% ±1.2%, 72.1% ±2.4%, 61.8% ±1.8%, 50.1% ±0.9% , respectively; The apoptosis rate increased gradually with the ratio of drug concentration were 8.5% ±0.8%, 11.7% ±1.4% , 24.7% ±1.8% , 31.2% ±2.3% , 35. 1% ±1.4% , respectively. Conclusion Lenalidomide can inhibit the growth of KM3 cells and induce the apoptosis of these cells, hence it plays the role of anti - cancer effect, and shows various degree of dosage dependent inhibition on KM3 cells.%目的 研究雷利度胺对多发性骨髓瘤(MM) 细胞KM3细胞凋亡的影响并初步探讨其作用机制.方法 用台盼蓝拒染法检测雷利度胺对KM3细胞生长抑制的影响,运用流式细胞仪(Annexin-V/FITC法)检测细胞凋亡.结果不同浓度(0 nmol/L、40 nmol/L、80 nmol/L、100 nmol/L、200nmol/L)的雷利度胺分别作用于KM3细胞24 h后,KM3 细胞的存活百分比逐渐减少,分别为100.0%±2.5%,87.2%±1.2%,72.1%±2.4%,61.8%±1.8%,50.1%±0.9%;细胞凋亡率随药物浓度的增加逐渐增加,分别为8.5%±0.8%、11.7%±1.4%、24.7%±1.8%、31.2%±2.3%、35.1%±1.4%.结论 雷利度胺抑制KM3 细胞的生长并诱导其发生细胞凋亡,而且不同浓度的雷利度胺对KM3细胞呈现剂量依赖性抑制作用.
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