慢性丙型肝炎患者中CD4+ CD25+ CD127-调节性 T 细胞的表达及意义

摘要

Objective To explore the expression of CD4 + CD25 + CD127 - regulatory T Cells from patients with chronic hepatitis C viral infection,and to evaluate its clinical significance. Methods 67 patients with HCV and 32 healthy volunteers were included in this study. The proportion of CD4 + CD25 + CD127 - regulatory T cells in peripheral blood was detected by using flow cytometry. HCV RNA detection in the pe-ripheral blood of HCV patients were done by real - time fluorescent quantitative PCR. Results Comparing with healthy controls,drug abusing and non - drug abusing HCV - infected patients in peripheral blood had a lower percentage of CD4 + CD25 + CD127 - regulatory T cells. The proportion of CD4 + CD25 + CD127 - regulatory T cells to CD4 + T cells in peripheral blood from drug abusing and non - drug abusing HCV - infected patients (4. 44% ± 1. 67% )account for(3. 84% ± 1. 95% )and(4. 44% ± 1. 67% )respectively,which was significantly lower than those from healthy controls(6. 10% ± 1. 65% )( P 0. 05);The expression of CD4 + CD25 + CD127 - regulatory T cells in HCV group before antiviral treatment was evident-ly lower than that in treated HCV group and control group( P < 0. 01). Conclusion The expression of CD4 + CD25 + CD127 - regulatory T cells in the antiviral treatment HCV patients was evidently higher than those of untreated HCV patients,which explained that the regulatory T cell possi-bly hold a role in the activation of the immune response to HCV infection. In addition,the expression level of CD4 + CD25 + CD127 - regulatory T cells in peripheral blood may be monitoring the patientˊs body cell immune status and become the index of body anti - virus immune response.%目的：探讨 CD4+ CD25+ CD127-调节性 T 细胞在慢性丙型肝炎（HCV）患者中的表达及其临床意义。方法收集 HCV 患者67例，并根据是否吸毒分为吸毒丙肝组35例和非吸毒丙肝组32例；健康体检人员32例作为对照。采用流式细胞仪检测患者外周血中 CD4+ CD25+ CD127-调节性 T 细胞的表达；应用实时荧光定量 PCR 的方法检测其HCV RNA 含量。结果慢性 HCV 患者中的吸毒感染组与非吸毒感染组的外周血中 CD4+ CD25+ CD127-调节性 T 细胞在 CD4+ T 细胞中所占比率分别为：3.84±1.95%、4.44±1.67%，明显低于对照组6.10±1.65%（ P <0.01）；而吸毒组与非吸毒组慢性 HCV 患者血液 CD4+ CD25+ CD127-调节性 T 细胞的表达值相比差异无统计学意义；HCV 患者抗病毒治疗前血液 CD4+ CD25+ CD127-调节性 T 细胞的表达值明显低于治疗后和正常对照组（ P <0.01）。结论 HCV 患者治疗前患者外周血 CD4+ CD25+ CD127-调节性 T 细胞的表达值较正常对照组明显降低，而在治疗有效后升高，说明CD4+ CD25+ CD127-调节性 T 细胞参与了 HCV 患者的机体免疫过程，而 CD4+ CD25+ CD127-调节性 T 细胞在外周血中的表达水平可能成为监测 HCV 患者体内细胞免疫状态和机体抗病毒免疫应答的指标。