广藿香油治疗皮肤光老化大鼠的效果研究及对p38 MAPK/ERK通路蛋白的调控作用分析

摘要

Objective The explore the effect of patchouli oil on photoaging skin rats and the involvement of p38MAPK/ERK signaling pathway. Methods Clean SD rats were divided into 6 groups. The control group rats were given normal light irradiation. The photoaging skin rats were duplicated by explore to UVA+VUB. PD98059 group and patchouli oil group rats were treated with corresponding drugs. The control group was given normal saline by intragastric administration. The expression of oxidation-reduction enzymes and p38MAPK/ERK related proteins were assayed. Results The results showed that the content of MDA,p38MAPK,Ras,Raf,MEK,ERK1/2,Bax,Caspase9,C-Fos and C-Jun were increased greatly( P ﹤0. 01)while the expression of Bcl2,SOD,GSHPX and CAT were decreased significantly( P ﹤0. 01)in model rats. The results also showed that these abnormal expressions were alleviated greatly after the medication of patchouli oil. At the mean time,the effect of patchouli oil had a dose dependent manner( P ﹤0. 01). Conclusion Patchouli oil exerts an significantly effect on the alleviation of photoaging skin rats which maybe related with the regulation of p38MAPK/ERK signaling pathway,Bax,Bcl2,C-Fos and C-Jun.%目的：对广藿香油治疗皮肤光老化大鼠的效果及对p38MAPK通路蛋白的调控作用进行分析，为临床治疗提供参考。方法48只清洁级SD大鼠随机分为6组：对照组、模型组、PD98059组和广藿香油低、中、高剂量治疗组，每组8只。对照组大鼠给予正常光照射，其余各组采用长波段紫外线+中波紫外线（ UVA+VUB）光源照射制备大鼠皮肤光老化模型。PD98059组和广藿香油组大鼠分别给予对应药物治疗，对照组给予生理盐水灌胃。分析各组大鼠血清中氧化还原酶、细胞凋亡蛋白及p38MAPK/ERK通路蛋白的表达。结果对照组皮肤无明显异常。模型组大鼠皮毛光泽度下降，皮肤颜色变深，且表皮干燥、粗糙、增厚、纹理增宽加深，弹性丧失；模型组大鼠血清中MDA、p38MAPK、Ras、Raf、MEK、ERK1/2、Bax、Caspase9及C-Fos和C-Jun水平明显升高而Bcl2、SOD、GSHPX和CAT蛋白水平明显降低（ P﹤0.01），而广藿香油治疗后上述蛋白异常得到明显的恢复，且与治疗前比较差异有统计学意义（ P ﹤0.01）；同时，广藿香油治疗具有剂量依从性，低剂量组、中剂量组和高剂量组之前差异有统计学意义（ P ﹤0.01）。结论藿香油治疗皮肤光老化大鼠的效果与其调控p38MAPK/ERK通路相关蛋白及Bax、Bcl2、C-Fos和C-Jun表达有关。