电针调控 p38MAPK 通路降低 AD 大鼠海马磷酸化 tau蛋白表达的研究

摘要

目的：通过测定海马内磷酸化 p38MAPK 和 tau 蛋白的表达，探讨针灸治疗阿尔茨海默病模型大鼠的作用机理。方法实验动物采用海马内注射 Aβ1-40复制 AD 动物造模，分为正常组、假手术组、模型组和电针组。电针组取百会穴和双侧肾俞进行电针治疗。Morris 水迷宫检测各组大鼠记忆能力，免疫组化、Western blot 检测大鼠海马内 p-p38MAPK 和 p-tau Thr181蛋白表达情况。结果电针组大鼠学习记忆能力显著提高，有统计学意义（P ＜0．05）；模型组大鼠海马内 p-p38MAPK 和 p-tau Thr181蛋白表达明显高于正常组，经电针治疗后表达显著减少，均有统计学意义（P ＜0．05）。结论针灸治疗可调控p38MAPK 通路，降低 AD 大鼠海马 p-tau 蛋白表达，改善学习记忆能力。%ABSTRACT:OBJECTIVE To explore the mechanism of treating Alzheimer's disease model rats by electro-acupuncture through detecting the phosphorylation of p38MAPK and tau protein in hippocampus.METHODS SD rats were randomly di-vided into control group,sham-operated group,model group and electric-acupuncture group.The model group and electric-ac-upuncture group were injected with Aβ1-40 into hippocampus.20 d later,the electric-acupuncture group had electric-acupuncture at Baihui(GV20) and Shenshu(BL23)appoints.Morris Water Maze was used to observe rates memory abilities,and western blot and immunohistochemistry were used to determine the expression of p-p38MAPK and p-tau181.RESULTS The electric-acupuncture group significantly improved learning and memory abilities(P <0.05);expression of p-p38MAPK and p-tau181 in the model group was significantly higher than the others,while significantly reduced after electric-acupuncture treatment(P <0.05).CONCLUSION Acupuncture can regulate expression of p-tau181 through p38MAPK signaling pathway and improve the learning and memory ability of AD rats.