热敏灸治疗儿童弱视的临床效果分析及对CREB和C-Fos蛋白的影响研究

摘要

目的：对热敏灸治疗儿童弱视的效果及对环磷腺苷效应元件结合蛋白（CREB）和 C - Fos 蛋白的影响进行研究，为临床治疗提供参考。方法对2010年1月至2015年12月期间诊治的儿童弱视160例（200眼）进行研究，分析患者经西医传统治疗（基础治疗组，100眼）或西医传统治疗联合热敏灸治疗（热敏灸治疗组，100眼）后的效果及对泪液中 CREB、蛋白激酶 A（PKA）、C - Fos、诱导型一氧化氮合酶（iNOS）、降钙素基因相关肽（CGRP）、血管内皮生长因子（VEGF）及转化生长因子β（TGF -β）和白细胞介素及脑源性神经生长因子（ BNDF）、缝隙连接蛋白（ Cx）30、Cx43及Cx45蛋白表达的影响。结果西医传统治疗或西医传统治疗联合热敏灸治疗均对弱视有明显的疗效，但热敏灸治疗组患者治疗有效率更高（ P ﹤0.05）；泪液中 CREB、PKA、C - Fos、iNOS、CGRP、VEGF 及 TGF -β 和白细胞介素水平明显降低而 BNDF、Cx30、Cx43及 Cx45蛋白水平明显升高，且与治疗前和基础治疗组相比差异有显著统计学意义（ P ﹤0.05）。结论热敏灸对儿童弱视有良好的治疗效果，其可能与调控 CREB 和 C - Fos 蛋白表达有关。%Objective The present research aimed to explore the effect of heat - sensitive moxibustion on childrenˊs amblyopia and the regulation of CERB and C - Fos proteins. Methods 160 cases of childrenˊs amblyopia subjects in our department from Jan 2010 to Dec 2015 were enrolled in this study. The effect of heat - sensitive moxibustion was assayed. The concentration of CREB and C - Fos were assayed. 100 healthy children were taken as normal control. Results The results showed that heat - sensitive moxibustion was effective in curing the childrenˊs amblyo-pia. The results also revealed that the concentration of CREB,PKA,C - Fos,iNOS,CGRP,VEGF interleukins and TGFβ were decreased( P﹤ 0. 05)significantly while the concentration of BNDF,Cx30,Cx43 and Cx45 were increased greatly( P ﹤ 0. 05). Conclusion Heat - sensi-tive moxibustion is effective in curing the childrenˊs amblyopia which may be related with the regulation of CREB and C - Fos.