稳定期慢性阻塞性肺疾病患者外周血T淋巴细胞活化标志物表达水平的研究

摘要

目的 通过研究慢性阻塞性肺疾病(COPD)稳定期患者外周血T淋巴细胞活化标志物的表达水平,探讨COPD患者的免疫功能状态.方法 前瞻性研究,纳入首都医科大学附属北京友谊医院COPD稳定期患者36例(COPD组),肺功能正常的吸烟者22例(吸烟组),体检正常无吸烟史者32例(正常对照组),采用流式细胞学方法,检测三组人群外周血T淋巴细胞亚群,以及外周血CD4十T淋巴细胞及CD8十T淋巴细胞CD69、CD38、人类白细胞抗原-DR(HLA-DR)的表达水平.结果 ①COPD组外周血T淋巴细胞各亚群的细胞数量及分布比率与吸烟组、正常对照组差异无统计学意义(P >0.05);②COPD组外周血CD4十T淋巴细胞CD38表达比率为3.60 ± 1.86%,HLA-DR表达比率为3.80 ± 2.88%;CD8十T淋巴细胞CD38表达比率为6.19 ± 3.33%,HLA-DR表达比率为1.97 ± 1.24%,均高于正常对照组及吸烟组(P 0.05). ②In PB of COPD patients,CD38 expression level on CD4十T cells was 3.60 ± 1.86%,HLA-DR expression level on CD4十T cells was 3.80 ± 2.88%;CD38 expression level on CD8十T cells was 6.19 ± 3.33%,HLA-DR expression level on CD8十T cells was 1.97 ± 1.24%;T cells activation markers including CD38 and HLA-DR expressing level are higher in COPD patients compare to the control group and smokers(P <0.05). ③T cells activation markers expressing level in COPD patients was positively correlated with smoking index(P <0.05). Conclusion There are more activated T cells in sta-ble COPD patients'PB compared with normal control group and smokers. COPD patients'cellular immune function status is more active than healthy population. And this kind of abnormal T-cell activation maybe related to long period smoke exposure.