Antigen-Specific Expression of Lymphocyte Activation Markers CD69, CD25, and HLA-DR in Patients with Immediate Reactions to Amoxicillin

摘要

We assessed the kinetic of the antigen-specific activation of T-cells in 36 patients with immediate reactions to amoxicillin and 16 controls, by the determination of the early activation marker CD69 and the late markers CD25 (IL2r) and HLA-DR. After in vitro incubation of the mononuclear cells isolated from peripheral blood with amoxicillin at 0, 4, 24, and 48 h, the cells were labeled with mononuclear antibodies: CD3 FITC, CD4 Percp Cy5.5, CD69 PE, CD25 PE, HLA-DR PE to be assessed subsequently by flowcytometry. Patients with immediate reactions to amoxicillin have an early activation of T CD4~+ cells that significantly increase the expression of CD69 and HLA-DR. Also we found a late activation with the increase of expression HLA-DR at 24 h. beta-lactam drags may induce both cellular and humoral allergic reactions, and there is evidence that T-cells play an important role in the patho-genesis of these reactions. In vitro T-cell response had been demonstrated in immediate and nonimmediate drug allergic reactions [1-4]. Amoxicillin is responsible for a major part of hypersensitivity reactions in drug allergy. While the exact mechanism is still a matter of debate, noncovalent drug presentation clearly leads to the activation of drag-specific T-cells [5-7]. The aim of this work was to assess the kinetic of the expression of early and delayed activation markers (CD69, CD25 and HLA-DR) in patients with immediate reactions to amoxicillin.