右美托咪定对胃腺癌SGC-7901细胞生长和转移的影响

摘要

Objective To observe different behavior of proliferation,migration and invasion of SGC-7901 cells when exposured to dexrnedetomidine of different concentrations.Methods Human gastric cancer cells SGC-7901 were inoculated on culture plate for 24 h,then were randomly divided into 5 groups:control group (group C),dexmedetomidine 312.5μg/ml group (group D1),dexme detomidine 625μg/ml group (group D2),dexmedetomidine 1 250 μg/ml group (group D3),dexmedetomidine 2 500 μg/ml group (group D4).Each group was medicated and incubated for 48 h,then the cell proliferation,migration and invasion immediately were detected by CCK-8 and Transwell.Results SGC-7901 cell viability of groups D1,D2,D3 和 D4 had no significant difference compared with that of group C.The invasion ability and migration ability of SGC-7901 cells in groups D1,D2,D3 and D4 were significantly higher than those in group C (P ＜ 0.05 or P ＜ 0.01).Conclusion Dexmedetomidine can promote migration and invasion of SGC-7901 cells.%目的 观察不同浓度右美托咪定对胃腺癌SGC-7901细胞增殖、迁移和侵袭的影响.方法 人胃腺癌SGC-7901细胞接种于培养板培养24 h,随机分为五组:对照组(C组)、右美托咪定312.5 μg/ml组(D1组)、右美托咪定625 μg/ml组(D2组)、右美托咪定1 250 μg/ml组(D3组)和右美托咪定2 500 μg/ml组(D4组).分别用不同浓度右美托咪定处理胃癌SGC-7901细胞48 h后,采用CCK-8法、Transwell法检测细胞增殖、侵袭和迁移.结果 与C组比较,D1、D2、D3和D4组SGC-7901细胞活力差异无统计学意义.D1、D2、D3和D4组SGC-7901细胞迁移能力明显高于C组(P＜0.05或P＜0.01),且呈剂量依赖性;D1、D2、D3和D4组SGC-7901细胞侵袭能力明显高于C组(P＜0.05或P＜0.01),且呈剂量依赖性.结论 右美托咪定可促进人胃癌细胞SGC-7901侵袭和迁移,对其增殖无明显影响.