Matriptase和HAI-1基因与前列腺癌关系的研究

摘要

Objective To investigate the expression and significance of Matriptase and HAI-1 protein in prostate cancer (CaP). Methods Specimens of 46 prostate cancers,20 benign prostate hyperplasias (BPH),10 high-grade intraepithelial neoplasias (PIN),and 10 normal prostates (NP) were used. Expressions of Matriptase and HAI-1 proteins in specimens were detected by SP of immunohistochemistry. The results were analyzed in relation to the clinicopathological data. Results The protein levels of Matriptase in CaP tissues were significantly higher than PIN tissues(Z＝-2.150,P＝0.032),and the expression of matriptase in CaP and PIN was higher than that in BPH and NP (Z＝-3.270,P＝0.001;Z＝-2.817,P＝0.005). No statistically significant difference was observed between BPH and NP group (Z＝-0.895,P＝0.325). A progressive increase in the protein levels of Matriptase was observed with increasing tumor grade (rs＝0.583,P＜0.01) and clinical stages（rs＝0.611,P＜0.01）in CaP specimens. The protein levels of HAI-1 in BPH and NP tissues were significantly higher than CaP and PIN tissues（Z＝-3.277,-3.315,P＜0.01）,the levels of HAI-1 in PIN were higher than CaP (Z＝-2.310,P＝0.020). No statistically significant difference was found between BPH and NP (Z＝-0.872,P＝0.330). A progressive decrease in the protein levels of HAI-1 was observed with increasing tumor grades（rs＝-0.634,P＜0.01） and clinical stages（rs＝-0.521,P＜0.01）. The expressions of Matriptase and HAI-1 in CaP tissues showed negative correlations（rs＝-0.712,-0.560,-0.465,respectively,P＜0.01）. Conclusions The abnormal expressions of Matriptase and HAI-1 proteins may be important events during the progression of CaP in humans. Matriptase and HAI-1 Protein may be used as parameters for assessing the malignancy and prognosis of CaP.%目的 检测matriptase与其抑制因子(hepatocyte growth factor activator inhibitor 1,HAI-1)在前列腺癌(Prostate cancers,CaP)发生发展中的表达变化及意义.方法 选取各种分化CaP组织46例,前列腺增生(benign prostate hyperplasias,BPH)20例,BPH并高级乳头状瘤(high-grade intraepithelial neoplasias,PIN)10例,正常前列腺(normal prostate,NP)组织10例,应用免疫组化SP法,检测比较各组织中Matriptase和HAI-1表达情况.结果 Matriptase表达在CaP组织高于PIN(Z＝-2.150,P＝0.032),两者均显著高于BPH和NP组(Z＝-3.270,P＝0.001;Z＝-2.817,P＝0.005).BPH和NP组间差异无统计学意义(Z＝-0.895,P＝0.325).CaP及PIN中HAI-1表达低于BPH和NP组(Z＝-3.277,-3.315,P均＜0.01),PIN表达高于CaP(Z＝-2.310,P＝0.020),BPH和NP组间差异无统计学意义(Z＝-0.872,P＝0.330);Matriptase表达与CaP病理分级、临床分期正相关(rs＝0.583,0.611,P均＜0.01),HAI-1表达与CaP病理分级、临床分期负相关(rs＝-0.635,-0.521,P＜0.01),组间差异均有统计学意义(P＜0.05).matriptase和HAI-1两者表达负相关,两者在BPH和NP、PIN、CaP组相关性逐渐降低(rs＝-0.712、-0.560、-0.465,P均＜0.01).结论 matriptase和HAI-1表达变化可能在CaP发生发展中具有重要意义,两者有望成为判断CaP恶性程度及预后的新型生物学标记.