胰岛素壳聚糖纳米粒的制备及影响包封率因素的考察

摘要

A new approach for the preparation of hydrophilic chitosan nanoparticles (CS-NPs), based on an ionic gelation process, was established which was extremely mild and involved the mixture of two aqueous phases at room temperature.One phase contained polysaccharide chitosan (CS) and polyethylene oxide(PEO ), and the other polyanion sodium tripolyphosphate(TPP). Size and zeta potential of the NPs could be modulated by varying the conditions for the preparation. The new NPs prepared in our experiment showed great entrapment efficiency of insulin up to 90% and various factors had an influence on it.This hydrophilic system might be a very promising carrier for administration of therapeutic proteins and other macromolecules that were susceptible to interact with CS.%采用离子感胶化法制备亲水性壳聚糖纳米粒.该法制备条件温和,是在室温下将两种水相混合而形成纳米粒.混合的两相中,其中一相含有壳聚糖和聚氧乙烯,另一相含有聚阴离子三聚磷酸钠.该纳米粒的粒度大小及ζ电位可以通过控制制剂处方组成及工艺进行调节.采用该法制备的胰岛素壳聚糖纳米粒,实验结果表明,胰岛素的包封率可高达90%,且制剂处方及工艺各因素对该纳米粒的包封率有不同程度的影响.该亲水性纳米粒可作为蛋白质或其它能与壳聚糖相互作用的大分子类药物给药系统的载体.