中药菲牛蛭化学成分的分析

摘要

Aim To determine and analyze fatty acids, amino acids and trace elements of Hirudinaria manillensis. Methods Contents of fatty acids, amino acids and trace elements were determined by GC/MS, HPLC and ICP-AES. Results H. manillensis contained sixteen fatty acids, among which the percentage of unsaturated fatty acids is 34.05%. The eighteen amino acids were detected, which included eight essential amino acids in human body with a ratio of 33.53%. There were eight trace elements (Ca,Cr, Cu, Fe, Mg, Mn, V and Zn) from H. manillensis, and the content of Ca, Zn and Fe were apparently higher than those of other elements. Conclusion These methods are simple and accurate. This study reports routine quantitative methods for the analysis of multiple components in H. manillensis by GC/MS, HPLC and ICP-AES.%目的 定性和定量分析菲牛蛭中的脂肪酸、氨基酸和微量元素成分.方法 采用气相色谱/质谱法、高效液相色谱法和电感偶合等离子发射光谱法,对菲牛蛭中的化学成分进行分析.结果 菲牛蛭中含有16种脂肪酸,其中不饱和脂肪酸占脂肪酸总量的34.05%.18种氨基酸,其中七种人体必需的氨基酸占所得氨基酸总量的28.16%.测得微量元素8种:Ca、Cr、Cu、Fe、Mg、Mn、V和Zn,其中Ca、Zn和Fe的含量明显高于其他微量元素的含量.结论 本文全面报道了菲牛蛭中各种化学成分的含量测定.