利用正交试验方法优化得到绿豆皮中总黄酮的最佳提取工艺.以芦丁为标准品,采用分光光度法在510 nm下对提取液中总黄酮含量进行测定.通过提取时间、乙醇体积分数、提取温度、固液比与提取次数5个因素的单因素试验,设计L_(16)(4~5)正交试验筛选最佳工艺.结果表明:提取时间150 min,乙醇体积分数50%,提取温度80 ℃,固液比1:10,提取次数2次,绿豆皮中总黄酮的提取量为3.879 mg/g,平均回收率为100.84%,精密度试验RSD为0.18%.%On the basis of single factor experiment, five influencing factors, namely extracting time, ethanol concentration, extraction temperature, material - liquid ratio and extraction times for extracting flavonoids from mung bean coat were established. Thus a L_(16)(4~5) orthogonal experiment was applied to optimize the extraction conditions, using spectrophotometry to determine the total flavonoids at 510nm wavelength. Results; The obtained optimal extraction conditions are extracting time 150 min, ethanol concentration 50% , extraction temperature 80 ℃, material -liquid ratio 1: 10 and extraction times 2 times. Under the conditions the extraction rate of flavonoids is 3.879 mg/g, the average recovery is 100.84% and RSD is 0.18%.
展开▼
