通过比较菜籽蛋白的各种提取方法,设计了以高温压榨菜籽粕为原料,采用多酶解法与碱提酸沉法相结合的新工艺提取菜籽粕蛋白质.在单因素试验的基础上,着重研究酶解过程,并用L9(34)正交优化试验得到了多酶酶解最佳工艺条件为:pH为9.0,温度为50℃,料液比为1∶10,多酶以α-淀粉酶+纤维素酶为宜,且加入量为α-淀粉酶90 U/g粕、纤维素酶45 U/g粕；在此最优条件下的验证实验所得蛋白质提取率为98.96％,菜籽蛋白产品的蛋白质含量为99.04％,产品为无特殊气味浅黄色的细晶粒和粉末固体.%By comparing a variety of rapeseed protein extraction method, we designed high - temperature pressed rapeseed as raw material and applied the new technology which combines multi - enzyme with alkaline extraction and acid precipitation to extract rapeseed meal protein. Based on the single - factor test, the study focused on the enzymatic process, and made L9 (3 ) orthogonal experiment to get the optimum conditions of multi - enzyme digestion : pH 9.0, temperature 50 ℃,solid - liquid ratio of 1:10. A multi - enzyme with α - amylase + cellulase was appropriate , and the added amount was α - amylase 90 U/g, and cellulase 45 U/g. Under the optimal conditions, the protein extraction ratio derived from verification experiment was 98. 96% ,and the protein content of rapeseed protein products was 99. 04%. The products were fine grain and light yellow powder solid with no special odor.
展开▼
