钙盐胁迫下苦马豆愈伤组织生理指标研究

摘要

To investigate the physiological response of callus of Swainsona salsula Taubert under salts stress, callus were induced from cotyledon explants on MS medium supplemented with 1.79 mg/L 2,4 - D and 1.15 mg/L Kt , and then subcuhured onto the same medium by adding the same concentration of NaCl 200 mmol/L） and different potassium salts at the same concentration （ [ Ca^2＋ ] = 150mmol - L^-1 ） for salts stress. After treated for 0,3,6,9 d, the contents of soluble proteins, the activities of SOD and POD, as well as the contents of praline in callus were tested. As a result, the content of soluble protein and the activity of SOD and POD of the callus from the medium with CaSO4 （150mmol · L^-1） showed the highest among all the test groups, but no obvious difference among them on praline content. The high activity of SOD was tested in callus from CaCI2 supplemented medium, however, the content of soluble protein and the activity of POD were relatively low. The content of soluble protein and the activity of SOD and POD of the callus from the medium with Ca（NO3）2 （150mmol · L^-1） were lower than other test groups, while the content of praline was found the highest. The results showed that the stress response of callus un- der salts from the medium with NaC1 （200 mmol· L^-1） and CaSO4（ 150mmol · L^-1） was more positive than oth- ers,and the weakest was callus on medium with Ca（NO3） 2- The results presented here make the basis for further investigating the mechanism of the response of S. salsula callus under salt stress and for cultivating mutant lines of this plant for high salt stress.%为了探讨在盐胁迫下苦马豆愈伤组织的生理效应，利用苦马豆子叶外植体在含有1．79mg／L2，4-D和1．15mg／LKt的Ms培养基上诱导的愈伤组织为材料，经添加相同盐浓度NaCl（200mmol／L）和在相同浓度（[Ca^2＋]=150mmol·L^-1）下的不同钙盐（CaCl2、Ca（NO3）2或CaSO4）胁迫处理，测定愈伤组织在不同时间（0，3，6，9d）胁迫处理后的可溶性蛋白含量、SOD和POD活性及脯氨酸含量．结果显示，CaSO。测试组中愈伤组织的可溶性蛋白含量，SOD活性，POD活性均为组间最高，脯氨酸含量与对照组无显著差异．CaCl2测试组中愈伤组织的SOD活性较高，可溶性蛋白含量和POD活性都较低，脯氨酸含量无显著差异．Ca（NO3）2测试组可溶性蛋白含量，SOD活性，POD活性均为组间最低，脯氨酸含量显著高于对照组和其余测试组．可以看出CaSO4胁迫组的积极响应度大于CaCl2及Ca（NO3）2组，Ca（NO3）2测试组最弱．研究结果为进一步研究苦马豆愈伤组织的耐盐机制以及培育抗性突变系奠定了基础．