目的 通过体外细胞培养法评价碳纤维增强碳与碳化硅双基体陶瓷基复合材料(C/C-SiC)对细胞生长和凋亡的影响.方法 用实验材料不同浓度浸提液培养小鼠成纤维细胞L929,采用MTT法检测细胞的相对增殖度;采用急性溶血试验检测材料对血细胞的溶血作用,计算溶血率;采用流式细胞仪、Annexin V-FITC/PI双染法检测阴性对照组、100%C/C-SiC组、纯钛组、阳性对照组的细胞散点图,计算正常细胞、早期凋亡、晚期凋亡和坏死细胞的比例.结果 C/C-SiC复合材料的细胞毒性为1级,溶血率为0.156%,无明显溶血反应,与阴性对照组和纯钛组的差异无统计学意义(P＞0.05).C/C-SiC组4个象限细胞比例与纯钛组和阴性对照组比较差异无统计学意义(P＞0.05),阳性对照组的早期凋亡、正常细胞比例与其他任一组比较差异均有统计学意义(P＜0.05).结论 C/C-SiC复合材料有生物安全性基础,无细胞毒性,无溶血反应.%Objective To evaluate the effect of C/C-SiC composite on the growth and apoptosis of mouse fibroblast cells. Methods Mouse fibroblasts (L929) were cultured in a series of elution of specimen, MTT assay was performed to investigate the relative growth rates;Hemolytic reaction of specimen to blood cells was detected by acute hemolysis test; Cell scatter diagrams of elutes of negative control group,100％C/C-SiC group,ti tanium group,positive control group was detected by Annexin V-FITC/PI double staining,the viable,early apoptotic,late apoptotic and necrosis cells were calculated. Results The cytotoxicity of C/C-SiC composite was grade 1,acute hemolysis rate was 0.156％. There were no significant differences between the negative control group and C/C-SiC group or between the titanium group and C/CSiC group (P ＞ 0.05 );The FACS images showed that the proportions of cells in four quadrants of titanium group,negative control group were not statistically different from C/C-SiC group (P ＞ 0.05),there were more early apoptotic cells and less viable cells in positive control group than that of other three groups (P ＜ 0.05). Conclusion The C/C-SiC composite was compatible with tested cells,with a relative background of biological safty.
展开▼
