替莫普利对大鼠血管平滑肌细胞弹性蛋白酶的影响

摘要

Objective To study the effect of temocapril on the level, expression, and activity of elastase in vascular smooth muscle cells (VSMC) in balloon-injured rat artery. Methods The rat model of arterial stenosis induced by artery injury was established. The Wistar rats were treated with temocapril hydrochloride (experimental group) and medicine solution (control group), respectively. On days 2, 3,and 5 after the artery injury, the percentages of cells positive for elastase Ⅲ B and elastase Ⅱ mRNA and the activity of elastase were determined in both groups. Collagenase in situ zymography was performed on day 3 after the artery injury. The intimal area was measured 10 days after the artery injury. Results Compared with the control group, the percentages of cells positive for elastase ⅢB and elastase Ⅱ mRNA,the activity of elastase, the level of type Ⅳ collagenase, and the intimal area significantly decreased in the experimental group. Conclusion Temocapril may inhibit intimal hypertrophy by inhibiting the level, expression, and activity of elastase and the level of collagenase in VSMCs in the injured artery.%目的 研究替莫普利对大鼠球囊损伤动脉血管平滑肌细胞(VSMC)的弹性蛋白酶水平、表达和活性的影响.方法 建立大鼠损伤动脉再狭窄模型,实验组给予盐酸替莫普利,对照组给予药品溶剂.动脉损伤后第2、3、5天测定两组VSMC的弹性蛋白酶ⅢB阳性细胞率;采用原位杂交的方法测定两组弹性蛋白酶ⅡmRNA阳性细胞率;测定两组弹性蛋白酶活性值.动脉损伤后第3天对两组进行原位明胶酶谱分析.动脉损伤后第10天测定两组内膜面积.结果 替莫普利组与对照组比较,大鼠损伤动脉VSMC的弹性蛋白酶ⅢB、弹性蛋白酶ⅡmRNA阳性细胞率显著降低,弹性蛋白酶活性显著降低;同时明胶酶水平显著降低,内膜面积显著减小.结论 替莫普利可能通过抑制大鼠损伤动脉VSMC的弹性蛋白酶水平、表达和活性及抑制明胶酶水平,抑制损伤动脉内膜肥厚.