腺病毒转染对树突状细胞成熟的影响

摘要

目的 研究腺病毒转染能否促进树突状细胞(DC)的成熟.方法 分别用复制缺陷型腺病毒空载体(Ad-null)和表达乳腺珠蛋白的重组腺病毒(Ad-MGBA)转染DC(Ad-null DC组和Ad-MGBA DC组),用添加和不添加肿瘤坏死因子(TNF-α)的DC作为对照组(TNF-α DC组),另设DC空白对照组.用相差显微镜观察DC的形态变化,用流式细胞术检测各组DC表面成熟标志CD80、CD83、CD86的变化,用ELISA检测各组DC上清中白细胞介素12(IL-12)的变化.结果 除DC空白对照组外,其余3组DC均表现出成熟DC的形态；转染DC组(Ad-null DC组和Ad-MGBA DC组)CD80、CD83、CD86、IL-12的表达水平均介于DC空白对照组和TNF-α DC组之间,在Ad-null DC组与Ad-MGBA DC组之间上述分子表达无统计学差异.结论 转染腺病毒载体能够促进DC的成熟,但促进DC成熟的作用低于TNF-α对DC促成熟的作用.%Objective To investigate the effects of recombinant replication-deficient adenovirus transfection on maturation of dentritic cells (DC).Methods DC were transfected with recombinant replication-deficient null adenovirus (Ad-null) and recombinant adenovirus encoding mammaglobin A(Ad-MGBA),respectively.Non-transfected DC and DC treated by TNF-α were used as controls.The morphological characteristics of DC were observed under a phase-contrast microscope.The cell surface expressions of CD80,CD83,and CD86 on DC were measured by flow cytometry (FCM).The level of IL-12 was detected by ELISA.Results Except for non-transfected DC,all the other three DC groups displayed maturation characters.The levels of CD80,CD83,CD86 and IL-12 on DC transfected with Ad-null and Ad-MGBA were between those of non-transfected DC and TNF-α treated DC.Conclusion Transfection with adenovirus alone can induce DC maturation,however,the stimulation effect is lower than TNF-α induction.