Objective To verify whether NGAL can be used to reflect the function of rat kidney undergoing different warm ischemia time. Methods Male SD rats were used to establish an ischemia reperfusion model. According to the length of warm ischemia time,rats were divided into control group (warm ischemia time for 0 minute), 15 minutes group (warm ischemia time for 15 minutes),30 minutes group( warm ischemia time for 30 minutnes),45 minutes group( warm ischemia time for 45 minutnes),60 minutes group (warm ischemia time for 60 minutes) . After reperfusion for 8 hours,allograft samples were collected and sectioned for HE,immunohistochemistry and Western blot, respectively. Blood were collected from the inferior vena cava for further tests. Results With the extension of the warm ischemia time,the kidney tubular became swollen and more necrotic. The expression of NGAL became stronger, serum Cr levels got higher. The difference of NGAL expression was found among control group, 15 minutes group,and 30 minutes group. Conclusion To reflect the rat renal function within 30 minutes of warm ischemia injury NGAL is more sensitive than Cr. Combines with pathological examination, NGAL could reflect function of kidney under warm ischemia injury better.%目的 建立大鼠肾脏缺血再灌注损伤模型,测定肾组织中中性粒细胞明胶酶脂质运载蛋白(NGAL)的表达情况,探讨其在反映肾脏热缺血损伤程度方面的应用价值.方法 建立大鼠肾脏的缺血再灌注损伤模型,根据热缺血时间将大鼠分为对照组(热缺血0 min组)、热缺血15 min、30 min、45 min和60 min组.再灌注8h后,HE染色观察肾脏病理学变化,免疫组化检测肾组织NGAL的表达,免疫印迹法检测胞质中NGAL的含量,下腔静脉抽血并测定血清肌酐(Cr)含量.结果 随着热缺血时间的延长,肾小管肿胀坏死程度加重,肾组织NGAL表达增强,血清Cr增高.对照组、热缺血15 min组和30 min组肾组织NGAL的表达有统计学差异.结论 与Cr相比,肾组织NGAL可更敏感、准确地反映热缺血30 min内大鼠肾功能情况,联合病理学检查则可更好的反映热缺血损伤对肾脏功能的影响程度.
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