Objective To investigate the mechanism by which resveratrol enhances adiponectin expression in 3T3-L1 cells. Methods 3T3-L1 cells were treated with vehicle, resveratrol, TNF-α, or combination of resveratrol and TNF-α; released adiponectin was measured by ELBA; adiponectin and PPAR-γ mRNA levels were analyzed using real time PCR;PPAR-γ activity was determined with PPAR-γ activity kit. Results Compared with vehicle-treated control, PPAR-γ and adiponectin expressions were enhanced and suppressed in differentiated 3T3-L1 cells treated with resveratrol and TNF-α, respectively. Compared with TNF-a alone treatment, combined treatment with resveratrol recovered TNF-α mediated suppression of adiponectin expression and release; resveratrol compromised the suppressive effect of TNF-α on mRNA expression and activity of PPAR-γ (P< 0.05). Conclusion Resveratrol recovered TNF-a mediated reduction of adiponectin expression and release via regulation of PPAR-γ activity.%目的 探讨白藜芦醇增加脂肪细胞3T3-L1中脂联素表达的作用机制.方法 设立空白对照组、白藜芦醇组、肿瘤坏死因子(TNF-α)组及白藜芦醇+TNF-α组.用白藜芦醇、TNF-α或者二者联合处理3T3-L1脂肪细胞.用酶联免疫吸附法测定脂联素水平；实时定量PCR测定脂联素和过氧化物酶体增生物激活受体(PPAR-γ)mRNA水平；用PPAR-γ转录因子试剂盒测定PPAR-γ活性.结果 与空白对照组比较,白藜芦醇组中PPAR-γ及脂联素mRNA在3T3-L1脂肪细胞中的表达增加(P＜0.05)；TNF-α组中脂联素mRNA表达及释放减少(P＜0.05).与TNF-α组比较,白藜芦醇+TNF-α组中脂联素mRNA的表达及分泌增加(P＜0.05)；白藜芦醇拮抗TNF-α对分化3T3-L1脂肪细胞中PPAR-γ mRNA表达及活性抑制(P＜0.05).结论 白藜芦醇能够通过调节PPAR-γ的转录活性继而拮抗TNF-α诱导下调3T3-L1脂肪细胞中脂联素的表达及分泌.
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