柴芩承气汤对急性胰腺炎大鼠肺泡巨噬细胞PERK基因表达的影响

摘要

目的 探讨柴芩承气汤(Chai Qin Cheng Qi decoction,CQCQD)对急性胰腺炎(acute pancreatitis,AP)大鼠肺泡巨噬细胞内质网双链RNA依赖蛋白激酶样ER激酶(PKR-like ER kinase,PERK)mRNA表达的影响.方法 将30只SD大鼠随机分成对照组、AP组及CQCQD组(n=10).AP组采用20%L-精氨酸腹腔注射(2.5 g·kg-1·1h-1×2)制作;CQCQD组分别于第0、2、4 h用CQCQD按0.2 mL/100 g灌胃,对照组采用生理盐水代替CQCQD灌胃,容积及方法与CQCQD组同.于6 h收集胰腺组织行病理检查,肺泡巨噬细胞检测PERK的mRNA表达,血清检测大鼠血清IL-6及TNF-α水平.结果 AP组胰腺组织病理评分(8.8±0.5)分,高于对照组(1.0±0.3)分及CQCQD组(5.4±0.6)分(P<0.05);CQCQD组血清IL-6及TNF-α(48.3±10.7 pg/mL,35.1±14.5 pg/mL)高于对照组(35.1±10.6 pg/mL,21.3±14.2 pg/mL,P<0.05),但低于AP组血清(67.5±13.1 pg/mL,52.1±13.7 pg/mL,P<0.05).与对照组比较,AP组巨噬细胞PERK mRNA表达上调,CQCQD灌胃可下调其表达(P<0.05).结论 CQCQD减少炎性因子释放可能与下调巨噬细胞内质网PERK mRNA表达有关.%Objective To investigate the effect of Chai Qin Cheng Qi Decoction (CQCQD)on the mRNA expression of PKR-like ER kinase (PERK)of the alveolar macrophage (AM)in rats with acute pancreatitis (AP). Methods Thirty Sprague-Dawley (SD)rats were randomised into the control group,AP group and CQCQD group, and each group consisted of 10 SD rats.The rats in the AP group were given intraperitoneal injection with 20%L-arginine (2.5 g·kg-1 ·1 h-1 ×2),those in the CQCQD group were treated with CQCQD 0.2 mL/100g by gavage for three times with 2-hour intervals every time,and the rats in the control group were treated with saline in the same way as those in the CQCQD group.After 6 hours of the treatment,the pancreatic tissues were collected for pathological examination,the alveolar macrophage was examined to detect the mRNA expression of PERK,and the serum was assayed to determine the levels of IL-6 and TNF-α.Results The pancreatic histopathologic score of the AP group (8.8±0.5)was significantly higher than that of the control group (1.0±0.3)and that of the CQCQD group (5.4±0.6)respectively (P<0.05).The levels of serum IL-6 and TNF-αin the CQCQD group (48.3±10.7 pg/mL,35.1±14.5 pg/mL)were significantly higher than those in the control group (35.1±10.6 pg/mL,21.3± 14.2 pg/mL)respectively (P<0.05),but they were significantly lower than those in the AP group (67.5±13.1 pg/mL,52.1±13.7 pg/mL)respectively (P<0.05).Compared with the control group,the expression levels of PERK mRNA in the AP group were significantly up-regulated (P<0.05),while those in the CQCQD group were significantly down-regulated after the treatment (P<0.05).Conclusion The mechanism of reducing the serum IL-6 and TNF-αby CQCQD may be correlated with the down-regulation of the mRNA expression of PERK in the alveolar macrophage.