Using catechin (CC) and oxazolidine Ⅱ (ZE) as the basic cross linking agents, the raw sheep skin collagen was treated. The influences of modify sequence, moisture content of the treated sample and kind of used solvents on the thermal stability of the samples were investigated by examining the shrinkage temperature of the modified collagen. According to the scanning electron microscope (SEM) images of samples before and after shrinkage, the histological morphology was observed, and the shrink mechanism of modified collagen was speculated by comparing its SEM images. The results show that the catechin-oxazolidine tanning sequence can enhance the heat resistance of the modified collagen, whose shrinkage temperature can be enhanced to 113.5℃.The moisture content of the sample has a negative effect on its thermal stability, and the thermal stability of the collagen will decrease with the increase of its moisture content. As to the solvent choosing, the acidity of the solvent will do a more destruction to the hydrothermal stability of the modified collagen, especially for collagen treated with CC-ZE sequence. It was found that, regardless of the tanning sequence, the fibers of non-shrunk sample are more evenly spreaded and compact than that of shrunk sample, and the regular structure of the collagen will be broken after heat treatment.%选用儿茶素和Ⅱ型噁唑烷为基本交联剂交联动物皮胶原,通过对交联后胶原热变性温度的考察探索了双组分交联剂的加入顺序、样品湿含量及溶剂处理对其热稳定性的影响;根据扫描电镜图像对热变性前后交联胶原的组织形态进行了观察,进而推测了交联胶原的热变性机理.结果发现:先儿茶素后噁唑烷顺序交联的胶原热稳定性较高,其热变性温度可达113.5℃;交联后胶原湿含量对其耐热稳定性有较大影响,在一定范围内胶原的热稳定性随着湿含量的增加而降低;交联后胶原的贮存环境的pH对其热稳定性影响较明显,pH为10.0时其热变性温度可降低多达20℃;无论何种顺序处理,热变性后的胶原纤维杂乱而分散地堆积在皮内.
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