报道了一种制备性能良好的温敏性纳米膜的简易方法。首先采用自由基聚合法将N-异丙基丙烯酰胺、甲基丙烯酸羟丙酯和甲基丙烯酸(3-三甲氧基硅)丙酯等原料合成为新型的PNIPAAm共聚物。通过动态光散射、傅里叶变换红外光谱、核磁共振氢谱和凝胶渗透色谱等对终产物的温敏特性、化学结构和分子量进行研究。进而采用旋转涂膜法和加热退火法在硅片或玻璃片上制备 PNIPAAm 共聚物纳米膜，并采用接触角测量仪、原子力显微镜和椭圆偏振仪等技术手段检测了不同制膜溶液浓度和测试温度条件下共聚物膜的表面润湿性、表面结构和总体厚度。结果表明所制备的PNIPAAm共聚物及其纳米膜均具有温敏特性，且可用于干细胞的有效收获。% This study reports an easy route for producing thermal responsive nanofilms with well defined film properties. A novel copolymer, poly(N-isopropylacrylamide-co-hydroxypropyl methacrylate-co-3-trimethoxysilypropyl methacrylate) (abbr. PNIPAAm copolymer), was synthesized by free radical polymerization. The thermal response, chemical structure and molecular weight of PNIPAAm copolymer were determined respectively by dynamic light scattering, Fourier transform infrared spectroscopy, 1H nuclear magnetic resonance and gel permeation chromatography. Then the fresh PNIPAAm copolymer nanofilms were fabricated on silicon wafers or glass coverslips by spin coating and thermal annealing, and their performances including surface wettability, surface structure and overall thickness under different conditions were also respectively tested by contact angle measurement, atomic force microscope and spectroscopic ellipsometry. The experimental results show that the PNIPAAm copolymer and its nanofilms with high thermo-sensitivity were successfully prepared. And the nanofilms can be used for effective recovery of stem cells.
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