河南周口番茄黄化曲叶病病原分子鉴定

摘要

In this paper, we identified the pathogen of tomato yellow leaf curl disease in Zhoukou of Henan Province (HN-ZK) by molecular method. The results showed that geminiviruses infecting tomato in Zhoukou was tomato yellow leaf curl virus (TYLCV), and the obtained sequence from HN-ZK isolate shared the highest similarity (98.7%) with that from Anhui AH-HB1 isolate. It was also indicated that HN-ZK isolate of TYLCV was most closely related to AH-HB1 isolate from the constructed phylogenetic tree. Thus, it was supposed that TYLCV isolate from Zhoukou was probably derived from Anhui.%　　应用分子生物学的方法鉴定了河南周口地区番茄黄化曲叶病的病原.鉴定结果表明，侵染河南周口番茄的双生病毒为番茄黄化曲叶病毒，与安徽AH-HB1分离物相似性最高，达到98.7%；从构建的系统关系树也可以看出，河南周口的番茄黄化曲叶病毒与安徽AH-HB1分离物的亲缘关系最近，推测河南周口番茄黄化曲叶病毒可能来源于安徽.