补肾健脾解毒利咽法对 IgA 肾病模型大鼠血清TNF-a、IL-6的影响

摘要

目的：探讨补肾健脾、解毒利咽法治疗 IgA 肾病（IgAN）的作用机制。方法将72只 Wistar 大鼠随机分为空白组，模型组，洛丁新组，补肾健脾、解毒利咽法高、中、低剂量组。采用口服 BSA 和尾静脉注射 LPS方法建立 IgAN 大鼠模型。观察给药8周后大鼠各时相尿红细胞计数、24 h 尿蛋白定量（UTP）、血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）的含量变化。结果与模型组相比，各组大鼠24 h UTP 均有所下降，其中中药中剂量组下降最为显著（P ﹤0.05）；中药各治疗组尿红细胞计数明显减少（P ﹤0.05），以中药中剂量组减少显著（P ﹤0.01）；洛丁新组尿中红细胞计数无下降（P ﹥0.05）。与空白组比较，模型组大鼠血清中 TNF-α、IL-6均明显升高，P ﹤0.05）。与模型组比较，治疗组大鼠血清中 TNF-α、IL-6明显减少（P ﹤0.05），以中药中剂量组疗效最显著（P ﹤0.01）。结论补肾健脾、解毒利咽法能降低 IgAN 模型大鼠血尿、蛋白尿，减少血清中TNF-α及 IL-6的含量。%Objective To study the effect and mechanism of Bushenjianpi and Jieduliyan method on IgA ne-phropathy. Methods The 72 Wistar rats were randomly divided into blank group,model group,Lotensin group,Bush-enjianpi and Jieduliyan high,medium and low dose group(is called traditional Chinese medicine high,medium and low dose group for short). The IgA nephropathy rat model were established by the method of feeding with bovine serum albumin(BSA)and injecting tail vein with lipopolysaccharide(LPS). Eight weeks administration later,each phase’s urine red blood cell count,UTP,TNF-αand IL-6 content were observed. Results Compared with model group,every groups’UTP decreased and traditional Chinese medicine dose group decreased extraordinary(P ﹤ 0. 05). Compared with model group,urine red blood cell count decreased significantly of every traditional Chinese medicine group(P ﹤0. 05),especially traditional Chinese medicine medium dose group improved significantly(P ﹤ 0. 01),but Lotensin group urine red blood cell count no drop(P ﹥ 0. 05). Compared with blank group,TNF-αIL-6 on model group’s in diabetic rats serum were significantly elevated(P ﹤ 0. 05). Compared with model group,TNF-α,IL-6 of treatment groups in diabetic rats serum decreased significantly(P ﹤ 0. 05),compared with every group,traditional Chinese medi-cine medium dose group’s data is the most significant(P ﹤ 0. 01). Conclusion Bushenjianpi and Jieduliyan method can reduce the model rats hematuria and proteinuria of IgA nephropathy,and reduce the content of IL-6 and TNF-αin serum which is better than Lotensin.