Electrochemical mechanism of rusticyanin （Rus.） isolated from A. ferrooxidans measured by Rus.-ZnS-QDs/L-Cys/Au electrode

摘要

Electrochimcal behaviors of rusticyanin (Rus.) isolated from Acidithiobacillus ferrooxidans were investigated through Rus.-ZnS-QDs/L-Cys/Au electrode.The cyclic voltammetric results indicate that rusticyanin immobilized on the surface of Rus.-ZnS-QDs/L-Cys/Au electrode can undergo a direct quasi-reversible electrochemical reaction.The immobilized rusticyanin is not denatured and still retains its activity in the temperature range of 19-43 °C.The reduction ability of the protein increases and its oxidation ability becomes weak with the increase of pH from 6.0 to 7.8.Fe2+ ions in the solution can promote the electron transfer kinetics of the immobilized rusticyanin and make its peak potentials (φp) markedly move negatively.