过表达白介素-10减少脂多糖所致大鼠星形胶质细胞炎症介质的释放

摘要

Objective To investigate the intervention effect of lentivirus expressing human IL-10 (LV-hIL-10) on activated astrocytes.Methods DI TNC1 cell line was treated with different concentrations of lipopolysaccharide (LPS) and time points.The expression of proinflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukinb-1β (IL-1 β) was detected by RT-PCR and ELISA.Moreover, the effect on the expression of proinflammatory cytokines TNF-α and IL-1β was analyzed in DI TNC1 cell lines infected with and without LV-hIL-10.Results The expression of TNF-α and IL-1β was increased in DI TNC1 induced by LPS.The expression of IL-10 was upregulated in DI TNC1 infected with LV-hIL-10.TNF-α and IL-1β were inhibited by IL-10 overexpression in DI TNC1 actived by LPS.Conclusion DI TNC1 is activated by LPS and secretes proinflammatory cytokines TNF-α and IL-1β as immune-like cells, and these activation is inhibited by hIL-10 overexpression.%目的:观察携带人IL-10基因的慢病毒(LV-hIL-10)对激活的星形胶质细胞的干预作用.方法:确定脂多糖(LPS)诱导DI TNC1细胞的最佳浓度和时间,利用表达白介素-10(IL-10)的慢病毒感染DI TNC1细胞,使用RT-PCR和ELISA法检测LPS诱导下DI TNC1细胞促炎因子TNF-α,IL-1β的表达改变.结果:LPS激活的DI TNC1细胞中,TNF-α和IL-1β的表达增加.LV-hIL-10感染DI TNC1细胞后,IL-10表达明显上调,LV-hIL-10感染LPS诱导的DI TNC1细胞后,明显抑制TNF-α,IL-1β mRNA的表达及其蛋白分泌.结论:LPS能激活星形胶质细胞产生免疫细胞样作用分泌TNF-α,IL-1β,而LV-hIL-10能抑制其分泌作用.