Differential Properties of Hucous Glycopreteins Produced by Allergic Inflammation and Lipopolysaccharide Stimulation in Rat Nasal Epithelium

摘要

To examine the differential properties of mucous gly-coproteins, we produced hyperplastic and metaplastic changes in goblet cells of rat nasal epithelium by intrana-sal instillation of ovalbumin (OVA) in OVA-sensitized rats, and by intranasal lipopolysaccharides (LPS) instillation. The epithelial mucosubstance was quantitatively examined by AB-PAS and lectin histochemistry.The newly produced mucin after OVA challenge or LPS instillation contained a high amount of sulfomucin and a low amount of neutral glycoprotein: LPS-induced mucin contained more sulfomucin (70.1% of total) and less neutral glycoprotein (8.6%) than OVA-induced mucin (sulfomucin 33.6%, neutral glycoprotein 41.8%). Four of the lectins, PNA, MAL II, SNA, UEA I, stained some of the mucosubstance, indicating the presence of galactose-N-acetylgaiactosamine, alpha2,3-linked and a2,6-linked sialic acid-galactose, and fucose residues. After LPS instillation, the reactivity was higher for galactose-N-acetylgalactosamine (64.8% of total) and alpha2,3-linked sialic acid-galactose (75.8%) than after saline instillation (3.5 and 19.1%, respectively) or OVA challenge (5.8 and 32.3%). A 2-fold increase in mucin mRNA (rat Muc5ac) expression was induced after LPS instillation or OVA challenge.