大鼠不同脑区线粒体中α-突触核蛋白含量与各种氧化和抗氧化指标的关系

摘要

Objective To study the relationship between mitochondrial o-synuclein (α-SYN) contents and oxidative stress levels in different rat brain regions and its association with selective damage of dopaminergic neurons in patients with Parkinson's disease.Methods Mitochondria were isolated using Percoll gradient centrifugation and mitochondrial α-SYN contents were measured using Western blotting and ELISA. Oxidative stress levels were evaluated by measuring oxidative malonaldehyde (MDA) and anti-oxidative indices total anti-oxidation capability ( T-AOC), glutathione ( GSH), glutathione peroxidase (GSH-PX) and superoxide dismutase ( SOD )in brain tissues. Results α-SYN contents were higher in PD vulnerable area such as olfactory bulb, striatum and hippocampus than in frontal cortex and cerebellum. Conclusion Mitochondrial α-SYN contents are different in various rat brain regions but it is not related to oxidative levels.%目的 分析大鼠不同脑区线粒体中α-突触核蛋白(α-synuclein,α-SYN)含量与氧化应激水平及其相互关系,探讨帕金森病(Parkinson's disease,PD)选择性损伤神经元的原因.方法 采用Percoll密度梯度离心法提取脑组织线粒体、Western blotting法和ELISA法测定线粒体中α-SYN含量;通过检测脑组织主要氧化指标丙二醛(malonaldehyde,MDA)和抗氧化指标总抗氧化能力(total anti-oxidation capability,T-AOC)、谷胱甘肽(glutathione,GSH)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-PX)和超氧化物歧化酶(superoxide dismutase,SOD)的含量判定氧化应激水平.结果 不同脑区线粒体中的α-SYN含量不同,其中,PD易损区纹状体、海马与嗅球中的线粒体中α-SYN的含量较高,而PD非易损区额叶皮质和小脑中的线粒体中α-SYN含量较低,易损区与非易损区线粒体中的α-SYN含量的差异有统计学意义.不同脑区各种氧化和抗氧化指标应有所不同,但差异无统计学意义,与线粒体中的α-SYN含量没有明显关系.结论 不同脑区线粒体中α-SYN含量不同,但与氧化应激水平无相关性.