淫羊藿苷对基因转染PC12细胞α-突触核蛋白过表达和泛素-蛋白酶体系统的影响

摘要

Objective To explore the effects of icariin, an effective component of Epimedium, on the over-expression and aggregation of α-synuclein (α-Syn) and other protein in vitro and mechanisms of the effects.Methods Different concentrations of icariin were incubated with α-Syn gene-transfected PC12 cells for 24 h.The change in α-Syn mRNA content in the cells was determined by RTPCR.The protein expression of α-Syn, UCH-L1 and Parkin was measured by Western blotting.Results Incubation of icariin(40 ～ 80 μmol/L) with α-Syn gene-transfected PC12 cells for 24 h significantly decreased α-Syn mRNA content, inhibited α-Syn protein expression and aggregation, and enhanced the protein expression of UCH-L1 and Parkin in the model cells.Conclusion Icariin inhibited the over-expression and aggregation of α-Syn in gene-tranfected PC12 cells in vitro, and the mechanisms may be related to the inhibition of synthesis and the enhancement of degradation of α-synuclein.%目的 观察淫羊藿苷在体外试验中对α-突触核蛋白(α-synuclein, α-Syn)及相关蛋白表达的影响及其作用机制.方法 不同浓度的淫羊藿苷与转染α-Syn基因PC12细胞共孵育24 h,应用RT-PCR法检测各组细胞α-Syn mRNA含量,Western免疫印迹法检测细胞中α-Syn、泛素化蛋白羧基端水解酶(Ubiquitin C-terminal hydrolase protein,UCH-L1)和Parkin蛋白的表达,观察淫羊藿苷对它们的影响.结果 淫羊藿苷浓度40～80 μmol/L与转染α-Syn基因细胞孵育24 h,能够明显减少模型细胞中α-Syn mRNA含量,抑制Syn蛋白表达和聚集,增强UCH-L1和Parkin蛋白表达.结论 淫羊藿苷在体外对基因转染细胞的α-Syn过表达和聚集有明显抑制作用,其作用机制与抑制α-Syn合成和增强α-Syn降解有关.