Bmi-1基因对人类胚胎纹状体来源神经干细胞凋亡的影响

摘要

目的：探讨Bmi-1基因对人类胚胎纹状体来源神经干细胞（neural stem cell，NSC）凋亡的影响。方法提取16～18周自然流产胚胎脑组织纹状体的NSC，体外培养至3～5代，用慢病毒载体对其进行Bmi-1基因的RNA干扰和过表达回复。观察Bmi-1基因对体外NSC凋亡的影响。结果在感染后2 h和3 d两个时间点，Bmi-1基因的RNA干扰显著增加了体外培养的NSC的凋亡率。结论 Bmi-1基因对于体外培养的NSC具有抑制其凋亡的作用，在分析该基因对NSC增生、自我更新等生物学属性的影响时，应当将Bmi-1基因的抗凋亡作用考虑在内。%Objective To explore the effect of Bmi-1 on the apoptosis of human fetal striatum derived neural stem cell (NSC ). Methods The NSC was isolated from the striatum of 1 6-1 8 week fetus suffered from accidental spontaneous abortion,cultured and passaged 3-5 times in vitro.Bmi-1 RNA interference and rescue (over-expression of Bmi-1 )experiments were performed by lentivirus.The effects of Bmi-1 on the apoptosis of NSC were then observed.Results The Bmi-1 transcription of human striatum derived NSCs was decreased significantly by RNAi and was reversed by rescue experiments(overexpression of Bmi-1 ).Correspondingly,both at the time points of 2h and 3d after passaging,the RNAi of Bmi-1 increased the apoptosis significantly.Conclusion Given human fetal striatum derived NSCs,Bmi-1 inhibited their apoptosis in vitro.In the cases studying the effect of Bmi-1 on the self-renewal,proliferation of NSC, the anti-apoptosis effect of Bmi-1 should be taken into consideration.