七氟烷与高浓度氧对老年小鼠海马caspase-3的表达及认知功能的影响

摘要

Objective To investigate the impact of sevoflurane and high concentration of oxygen on expression of caspase-3 and cognitive function of aged mice.Methods Thirty three healthy male C57/BL6 elderly mice (14 months) were randomly divided into three groups:group of high concentration of oxygen (HO group,n =11):inhaling 100％ oxygen for 4 h;high concentration of oxygen + sevoflurane group (HS group,n =11):taking in 100％ oxygen with 2.5％ sevoflurane for 4 h;control group (Con group,n =11):taking in air for 4 h.Cognitive function in the elderly mice was tested in the Morris water maze.Western blotting is used to test the expression of caspase-3 in hippocampus.Results Compared with Group Con,Group HO saw no difference in numbers of crossing the platform and Caspase-3 expression.Group HS crossed the platform less (P =0.023),and the caspase-3 expression of the group increased significantly(P =0.036).Conclusion Inhaling 100％ oxygen had no impact on cognitive function in the elderly mice;Inhaling 2.5％ Sevoflurane with 100％ oxygen could increase expression of caspase-3,and it had close relations with the cognitive dysfunction of the aged mice.%目的 探讨七氟烷与高浓度氧对老年小鼠海马半胱氨酸蛋白酶-3(caspase-3)的表达及认知功能的影响.方法 健康雄性C57/BL6老年小鼠(14月龄)33只,采用数字表法将其随机分为3组:高浓度氧组(HO组,n=ll):吸100％(体积分数)氧4h;高浓度氧+七氟烷组(HS组,n=11):吸100％(体积分数)氧+2.5％(体积分数)七氟烷4h;对照组(Con组,n=11):吸空气4h.24 h后采用Morris水迷宫法研究3组小鼠麻醉后连续5d找到平台所用的时间(逃避潜伏期)和第5天去除平台后,90 s内穿过原平台的次数.利用蛋白质印记(Western blotting)法检测小鼠海马caspase-3的表达.结果 与Con组相比,HO组的caspase-3表达差异无统计学意义(P＞0.05);HS组的穿越平台次数降低(P =0.023),caspase-3表达显著增加(P =0.036).结论 吸入100％(体积分数)氧对老年小鼠认知功能无影响,2.5％(体积分数)七氟烷与100％(体积分数)氧同时吸入增加老年小鼠海马caspase-3的表达,与老年小鼠产生认知功能障碍有关.