手术创伤引起的海马炎症反应对老年小鼠术后认知功能的影响

摘要

目的:探讨手术创伤引起的海马炎症反应对老年小鼠术后认知功能的影响.方法:96只雄性昆明小鼠随机分为对照组(NS组)、麻醉组(Ane组)、假手术组(Sham组)和手术组(Sur组)4组(n均为24).使用Morris水迷宫进行认知功能测试.测试完毕后,采用Western blot和Real Time RT-PCR检测海马IL-1β、IL-6、TNF-α蛋白及mRNA表达水平.结果:术后第1、3、7天,4组动物目标象限停留时间百分比及1 min内穿台次数差异有统计学意义(P均<0.05),且Sur组低于其他3组;4组动物海马IL-1β、IL-6、TNF-α 蛋白及mRNA的表达差异有统计学意义(P均<0.001),Sur组较其他3组表达上调.结论:手术创伤能够引起老年小鼠术后认知功能障碍,其机制可能与术后海马组织中IL-1β、IL-6及TNF-α的表达上调有关.%Aim;To investigate the effects of neuroinflammation in hippocampus triggered by surgical trauma on cogni -tive function in aged mice. Methods:A total of 96 male Kunming mice were randomly allocated into four groups (24 mice per group) : control group (group NS) , anesthesia group ( group Ane) , sham group ( group Sham) and surgery group ( group Sur). Cognitive function was assessed in Morris water maze . Then the hippocampus was immediately harvested for determi -nation of the protein and mRNA expression of IL -1β, IL-6, and TNF-α by Western blot and Real Time RT-CR. Results: On the 1st,3rd,and 7th day after treatment there were significant differences in the percent time spent in target quadrant and the number of platform-site crossovers within 1 min among the 4 groups (P < 0. 05 ) , and the 2 parameters were lower in group Sur than the other 3 groups. On the 1st,3rd and 7th day,there were significant differences in protein and mRNA ex -pression of IL-1β, IL-6, and TNF-α among the 4 groups (P < 0. 001 ) , and the above parameters was upregulated in group Sur than the other 3 groups. Conclusion: Postoperative cognitive dysfunction induced by surgical trauma in aged mice may be associated with up -regulation of IL -lβ, IL-6, TNF-α expression in hippocampus.