空肠弯曲菌Penner O:19 galE基因真核表达质粒的构建

摘要

目的 构建空肠弯曲菌Penner O:19 galE基因真核表达质粒.方法 PCR扩增空肠弯曲菌Penner O:19 galE基因,构建pGEM-T-galE克隆质粒及构建pEGFP-N1 -galE真核表达质粒,并检测真核表达质粒在293T细胞中的表达.结果 构建了真核表达质粒pEGFP-N1-galE,检测到其在293T细胞表达.结论 pEGFP-N1可作为Penner O:19 galE基因的真核表达载体.%Objective Construct eukaryotie expression recombinant plasmid of galE gene of campylobacter jejuni. Methods Cloned the gene galE of C. jejuni Penner 0: 19 , constructed the pGEM-T-galE plasmid and pEGFP-Nl-galE eularyotic expression recombinant plasmid, the expression of pEGFP-Nl-galE was investigated by transfected into cell 293T. Result pEGFP-Nl-galE eukaryotic expression plasmid was constructed successfully and expressed in 293T cell. Conclusion pEGFPNl eukaryotic expression plasmid is a vector of galE.