microRNA －17过表达对胶质瘤患者预后的影响

摘要

Objective To investigate the correlation of expression of miR -17 in glioma cells and prognosis of glioma patients.Methods qRT-PCR was used to determine the expression level of miR -17 in 108 glioma samples and 20 non-neoplasma brain samples.Kaplan-Meier test was used to investigate the relationship between expression of miR-17 and prognosis of glioma patients.Rseults Comparing to control group, the expression of miR-17 was significantly increased in glioma tissues ( P<0.001), and higher expression level of miR -17 was found in glioma tissues of higher WHO grade (P<0.01).The results of K-M analysis and Cox regression analysis showed that high expression of miR-17 was an independent factor for poor prognosis (P<0.01) and was significantly related to the poor prognosis of glioma patients.Conclusion This study shows that high expression of miR -17 could indicate poor prognosis of glioma patients.miR-17 may promote the development of glioma and could be a therapeutic target .%目的：探讨微小 RNA－17（miR－17）在胶质瘤细胞中的表达与人脑胶质瘤患者预后的相关性。方法实时定量 PCR（qRT－PCR）分析 miR－17在108胶质瘤组织和20正常脑组织中的表达情况。采用Kaplan－Meier 法分析 miR－17过表达胶质瘤患者预后的关联性。结果与正常脑组织比较中，miR－17在胶质瘤组织中的表达显著增高（ P＜0．001），且 miR－17在胶质瘤的表达水平随肿瘤病理分级的增高而增高（P＜0．01）。 Kaplan－Meier 生存和 Cox 回归分析显示，miR－17高表达是预测胶质瘤预后不良的独立因素（P＜0．01）。此外，分析还显示 miR－17的高表达与胶质瘤患者整体不良预后显著相关（P＜0．001）。结论本研究显示，miR－17高表达量可能对预测胶质瘤患者的不良预后具有潜在价值，表明了 miR－17高表达可能有助于脑胶质瘤的进展，并成为将来生物治疗的靶点。