小鼠心脏发育相关基因调控网络的生物信息学分析

摘要

Several key genes(Nkx2.5,Mef2c,Gata4/5/6,Tbx5 and Hand1) play the central role in vertebrate cardiac development. In this paper, six core genes ( Nkx2.5,Mef2c,Cata4,Cata6,Tbx5 and Hand1) were analyzed deeply in mouse by bioinformatic methods in order to get the cardiac developmental GRNs. Compared with the GRNs from literature, the position of Nkx2.5 and Cata4 are the same, acting as regulators regulate many other transcriptional factors. That Mef2c and Handl are regulated by other transcriptional factors is also the same between two networks. But there are two differences in the network of Tbx5. This research indicates that bioinformatics analysis can guide specific experimental study to a certain extent.%Nkx2.5,Mef2c,Gata4/5/6,Tbx5和Hand1等基因作为脊椎动物心脏发育相关基因调控网络的核心基因,对脊椎动物的心脏发育起核心调控作用.本研究主要通过对小鼠心脏发育相关核心基因的生物信息学分析,研究Nkx2.5,Mef2c,Gata4,Gata6,Tbx5和Hand1等核心转录因子之间的相互调控关系,得到小鼠心脏发育的核心基因调控网络(GRNs).与文献中总结的小鼠的GRNs作比较,发现Nkx2.5在小鼠心脏发育基因调控网络中的核心地位没有改变,并且Nkx2.5在网络中主要扮演了调控者的角色,它直接调控很多转录因子.Gata4是另一个主要起调控者作用的转录因子.另外小鼠的Mef2c和Hand1处于被多个转录因子调控的地位.变化比较大的是Tbx5所涉及的调控网络,主要有两点:Nkx2.5对Tbx5的表达有没有调控作用以及Tbx5对自身的表达有没有调控作用.研究表明生物信息学分析对具体实验研究有一定方向性指导意义.