神经细胞黏附分子在乳腺癌发生发展中的作用

摘要

Neural cell adhesion molecule(NCAM), a kind of glycoproteins expressing on the cell surface, engages in both cell-cell and cell-extracellular matrix interactions.It also influences cell adhesion, migration and proliferation.First, NCAM expression was compared in normal breast cells and malignant breast tumor cells.It was detected that the expression level of NCAM-140 in malignant breast cancer cells was higher than normal breast cells.When normal breast cells underwent epithelial to mesenchymal transition ( EMT) ,NCAM expression was upregulated.The expression level of E-cadherin in normal breast cells after stable transfection with NCAM-140 was decreased, N-cadherin was increased, cell motility was enhanced, and EGFR and STAT3 signal pathways were acti-vated.This study demonstrated that NCAM could promote EMT process, and provided a significant basis that NCAM played an impor-tant role in occurrence and development of breast cancer.%神经细胞黏附分子（ Neural cell adhesion molecule， NCAM）是一种定位在细胞膜上的糖蛋白，它能介导细胞与细胞及细胞与细胞外基质间相互作用，影响细胞黏附、迁移以及增殖等。首先，对比研究NCAM在正常乳腺细胞和恶性乳腺肿瘤细胞中的表达情况，发现恶性肿瘤细胞NCAM表达水平明显高于正常细胞；当正常乳腺细胞发生上皮间质转化（Epithelial－mesenchymal transition， EMT）后，NCAM表达水平上调；正常乳腺细胞稳定转染NCAM－140后，E－cadherin表达水平下调，N－cadherin表达水平上调，细胞运动能力明显增强，且激活EGFR和STAT3信号通路。说明了NCAM能够促进细胞发生EMT过程，揭示NCAM在乳腺肿瘤发生发展中起到的重要作用。